Candidiasis is the most common fungal infection affecting hospitalized patients, especially immunocompromised and critical patients. Limitations regarding the assertive diagnosis of both Candidemia and Candidiasis not only impairs the introduction of effective treatments but also lays a heavy financial burden over the health system. Furthermore, it is still challenging to ascertain whether diagnostic methods are accurate and whether treatment is effective for patients with Candidemia. These constraints come from the uncertainty of the pathophysiological mechanism by which the pathogen establishes the opportunistic infection. Additionally, it is the reason why some patients present positive blood culture results, and others do not, and why it is very difficult during clinical routines to prove Candidemia or invasive candidiasis. Taking into account the current situation, this contribution proposes two markers that may help to understand the mechanisms of infection by the pathogen: Leukotriene F4 and 5,6-dihydroxy-eicosatetraenoic. These two lipids putatively modulate the host's immune response, and the initial data presented in this contribution suggest that these lipids allow the opportunistic infection to be installed. The study was carried out using an omics-based platform using direct-infusion high-resolution mass spectrometry and allied with bioinformatics tools to provide accurate and reliable results for biomarker candidates screening.

念珠菌病是最常见的真菌感染，会影响住院患者，尤其是免疫功能低下和重症患者。关于对念珠菌病和念珠菌病的主观诊断的局限性不仅损害了有效治疗的引入，而且给卫生系统带来了沉重的经济负担。此外，确定诊断方法是否正确以及对念珠菌血症患者的治疗是否有效仍然具有挑战性。这些限制来自病原体建立机会感染的病理生理机制的不确定性。此外，这也是为什么某些患者的血液培养结果呈阳性，而其他患者的结果却不阳性的原因，以及为什么在临床常规过程中很难证明念珠菌血症或浸润性念珠菌病。考虑到目前的情况，该贡献提出了两个标志物，可能有助于了解病原体的感染机制：白三烯F4和5,6-二羟基-二十碳四烯酸。推测这两种脂质可调节宿主的免疫反应，并且此贡献中显示的初始数据表明，这些脂质允许安装机会性感染。该研究是使用基于组学的平台进行的，该平台使用直接输注高分辨率质谱仪，并与生物信息学工具联合使用，可为生物标志物候选物筛选提供准确可靠的结果。推测这两种脂质可调节宿主的免疫反应，并且此贡献中显示的初始数据表明，这些脂质允许安装机会性感染。该研究是使用基于组学的平台进行的，该平台使用直接输注高分辨率质谱仪，并与生物信息学工具联合使用，可为生物标志物候选物筛选提供准确可靠的结果。推测这两种脂质可调节宿主的免疫反应，并且此贡献中显示的初始数据表明，这些脂质允许安装机会性感染。该研究是使用基于组学的平台进行的，该平台使用直接输注高分辨率质谱仪，并与生物信息学工具联合使用，可为筛选生物标志物提供准确可靠的结果。