Titanium implants are widely used in dental and orthopedic surgeries. Osseointegration phenomena lead to direct contact between bone tissue and the implant surface. The quality of the bone-implant interface (BII), resulting from the properties of newly formed bone, determines the implant stability.

This study investigates the BII properties using a dedicated in vivo implant model consisting of a coin-shaped Ti-6Al-4V implant inserted in a rabbit femur for 10 weeks. A gap created below the implant was filled with newly formed bone tissue after healing. The properties of mature and newly formed bone tissues were compared using: i) Raman spectroscopy to assess the nanoscale compositional bone properties and ii) nanoindentation to quantify microscale elastic properties in site-matched regions. The mineral-to-matrix ratio, crystallinity (mineral size and lattice order), and the collagen cross-link ratio were significantly lower in newly formed bone tissue (e.g., a mineral-to-matrix ratio of 9.3 ± 0.5 for proline 853 cm⁻¹) compared to mature bone (15.6 ± 1). Nanoindentation measurements gave Young's modulus of 12.8 ± 1.8 GPa for newly formed bone and 15.7 ± 2.3 GPa for mature bone. This multimodal and multiscale approach leads to a better understanding of osseointegration phenomena.

钛植入物广泛用于牙科和骨科手术。骨整合现象导致骨组织与种植体表面直接接触。由新形成的骨的特性产生的骨 - 种植体界面 (BII) 的质量决定了种植体的稳定性。

本研究使用专用的体内植入物模型研究 BII 特性，该模型由插入兔股骨 10 周的硬币形 Ti-6Al-4V 植入物组成。愈合后新形成的骨组织填充了植入物下方的间隙。使用以下方法比较成熟和新形成的骨组织的特性：i) 拉曼光谱来评估纳米级的骨组成特性，以及 ii) 纳米压痕以量化位点匹配区域中的微米级弹性特性。新形成的骨组织中的矿物质与基质比、结晶度（矿物质大小和晶格顺序）和胶原交联比显着较低（例如，脯氨酸 853 cm 的矿物质与基质比为 9.3 ± 0.5 ⁻¹) 与成熟骨 (15.6 ± 1) 相比。纳米压痕测量给出的杨氏模量对于新形成的骨为 12.8 ± 1.8 GPa，对于成熟骨为 15.7 ± 2.3 GPa。这种多模式和多尺度方法有助于更好地理解骨整合现象。