Gene ( IF 3.5 ) Pub Date : 2020-07-21 , DOI: 10.1016/j.gene.2020.144968 Shaomei Li 1 , Wenshuo Chen 1 , Xinting Zheng 1 , Zhiwei Liu 1 , Ge Yang 1 , Xuewen Hu 1 , Chunyan Mou 1
The hair follicle is an excellent mini-system illustrating the mechanisms governing organogenesis and regeneration. Although the general mechanisms modulating skin and hair follicle development are widely studied in mouse and chicken models, the delicate network regulating skin and hair diversity remains largely unclear. Sheep is an additional model to address the various wool characteristics observed in nature. The coarse and fine wool sheep with diverse fibers were examined to show differences in the primary wool follicle size and skin thickness. The molecular dynamics in skin staged at the primary wool follicle induction between two sheep lines were investigated by RNA-sequencing analyses to generate 1994 differentially expressed genes revealing marker genes for epithelium (6 genes), dermal condensate (38 genes) and dermal fibroblast (58 genes) highly correlated with skin and wool follicle morphological differences. The DEGs were enriched in GO terms represented by epithelial cell migration and differentiation, regulation of hair follicle development and ectodermal placode formation, and KEGG pathways typified by WNT and Hedgehog signaling pathways governing the differences of skin structure. The qPCR detection of 9 genes confirmed the similar expression tendency with RNA-sequencing profiles. This comparative study of coarse and fine wool sheep skin reveals the presence of skin and wool follicle differences at primary wool follicle induction stage, and indicates the potential effectors (APCDD1, FGF20, DKK1, IGFBP3 and SFRP4) regulating the skin compartments during the early morphogenesis of primary wool follicles to shape the variable wool fiber thickness in later developmental stages.
中文翻译:
对粗和细羊毛绵羊皮的比较研究表明,早期的皮肤和羊毛多样性调节剂。
毛囊是一个极好的微型系统,阐明了控制器官发生和再生的机制。尽管在小鼠和鸡模型中广泛研究了调节皮肤和毛囊发育的一般机制,但调节皮肤和毛发多样性的精细网络仍然不清楚。绵羊是解决自然界中各种羊毛特征的另一种模式。检查具有不同纤维的粗毛羊和细毛羊,以显示其初级毛囊大小和皮肤厚度的差异。通过RNA测序分析研究了在两个绵羊系之间初次毛囊诱导时皮肤中的分子动力学,产生了1994个差异表达的基因,揭示了上皮的标记基因(6个基因),真皮凝结物(38个基因)和真皮成纤维细胞(58个基因)与皮肤和毛囊的形态学差异高度相关。DEG富含GO术语,代表上皮细胞迁移和分化,调节毛囊发育和外胚层斑形成,以及以WNT和Hedgehog信号通路为代表的KEGG通路控制皮肤结构的差异。9个基因的qPCR检测证实了与RNA测序谱相似的表达趋势。这项对粗羊毛和细羊毛绵羊皮的比较研究揭示了在初级毛囊诱导阶段皮肤和毛囊差异的存在,并指出了潜在的效应子(DEG富含GO术语,代表上皮细胞迁移和分化,调节毛囊发育和外胚层斑形成,以及以WNT和Hedgehog信号通路为代表的KEGG通路控制皮肤结构的差异。9个基因的qPCR检测证实了与RNA测序谱相似的表达趋势。这项对粗羊毛和细羊毛绵羊皮的比较研究揭示了在初级毛囊诱导阶段皮肤和毛囊差异的存在,并指出了潜在的效应子(DEG富含GO术语,代表上皮细胞迁移和分化,调节毛囊发育和外胚层斑形成,以及以WNT和Hedgehog信号通路为代表的KEGG通路控制皮肤结构的差异。9个基因的qPCR检测证实了与RNA测序谱相似的表达趋势。这项对粗羊毛和细羊毛绵羊皮的比较研究揭示了在初级毛囊诱导阶段皮肤和毛囊差异的存在,并指出了潜在的效应子(9个基因的qPCR检测证实了与RNA测序谱相似的表达趋势。这项对粗羊毛和细羊毛绵羊皮的比较研究揭示了在初级毛囊诱导阶段皮肤和毛囊差异的存在,并指出了潜在的效应子(9个基因的qPCR检测证实了与RNA测序谱相似的表达趋势。这项对粗羊毛和细羊毛绵羊皮的比较研究揭示了在初级毛囊诱导阶段皮肤和毛囊差异的存在,并指出了潜在的效应子(APCDD1,FGF20,DKK1,IGFBP3和SFRP4)在初级毛囊的早期形态发生过程中调节皮肤区室,以在发育后期形成可变的羊毛纤维厚度。