To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed. Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH₄)₂SO₄ concentration of 24 % (w/w) and extraction time of 15 min. Under such condition, Y_S (the recovery of polysaccharides) was 80.57 % with 90.40 % of Y_P (the recovery of proteins). In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios. Moreover, NFP-MAATPS exhibited stronger antioxidant activity in vitro and more potent anti-inflammatory activity in zebrafish embryos than NFP-HWE. Our results suggest that purified NFP-MAATPS could be potentially used in complementary medicines or functional foods.

为了优化从羌活多糖提取franchetii羌活（NFP），微波辅助水性两个相体系（MAATPS）和响应面分析法（RSM）被使用。结果表明，MAATPS的最佳条件如下：乙醇浓度为32％（w / w），（NH ₄）₂ SO ₄浓度为24％（w / w），提取时间为15分钟。在这种条件下，Y _S（多糖的回收率）为80.57％，Y _P为90.40％（蛋白质的回收）。此外，在最佳条件下对提取多糖（NFP-MAATPS）的理化性质进行分析表明，与传统提取方法（NFP-HWE）提取的多糖相比，NFP-MAATPS提取效率更高，平均分子量降低，不同单糖的组成和摩尔比。此外，与NFP-HWE相比，NFP-MAATPS在体外表现出更强的抗氧化活性，并在斑马鱼胚胎中表现出更强的抗炎活性。我们的结果表明，纯化的NFP-MAATPS可以潜在地用于辅助药物或功能食品。