当前位置: X-MOL 学术Bioelectrochemistry › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Electroporation of a hybrid bilayer membrane by scanning electrochemical microscope.
Bioelectrochemistry ( IF 5 ) Pub Date : 2020-07-21 , DOI: 10.1016/j.bioelechem.2020.107617
Aušra Valiūnienė 1 , Inga Gabriunaite 1 , Margarita Poderyte 1 , Arunas Ramanavicius 2
Affiliation  

A novel method, suitable for targeted electroporation of hybrid bilayer membranes (hBLMs) by scanning electrochemical microscope (SECM) is introduced by this work. A redox-probe-free system was applied for (i) SECM-based electroporation of a hBLM and for (ii) SECM-based visualization of pores formed by SECM-based electroporation in the hBLM. The hBLM was formed on a glass substrate modified by fluorine-doped tin oxide, and the structure (glass/FTO/hBLM) was used for further investigations. A specific ‘constant-current region’ at 1–30 µm distances between the UME and the hBLM surface was observed in the approach curves, which were registered while a Pt-based ultramicroelectrode (UME) was approaching the glass/FTO/hBLM surface. This ‘constant-current region’ was used as the characteristic feature for characterisation of the hBLM, and by assessment of the approach curves it was possible to distinguish whether an area of the hBLM was electroporated. SECM-based electroporation of the hBLM was performed by using increased potential difference between the reference electrode and the UME. Depending on the duration of the applied potential-pulse and on the distance between the UME and the hBLM surface, irreversible or reversible electroporation of the hBLM was achieved. The data shows that SECM can be successfully applied for both electroporation and characterisation of the hBLM.



中文翻译:

通过扫描电化学显微镜对双层膜进行电穿孔。

通过这项工作,提出了一种适用于通过扫描电化学显微镜(SECM)对杂化双层膜(hBLM)进行靶向电穿孔的新方法。将无氧化还原探针的系统应用于(i)hBLM的基于SECM的电穿孔,以及(ii)hBLM中基于SECM的电穿孔形成的孔的基于SECM的可视化。在掺氟氧化锡改性的玻璃基板上形成hBLM,并将其结构(玻璃/ FTO / hBLM)用于进一步研究。在进近曲线中观察到了UME与hBLM表面之间1–30 µm距离处的特定“恒定电流区域”,该曲线在Pt基超微电极(UME)接近玻璃/ FTO / hBLM表面时记录下来。该“恒定电流区域”被用作表征hBLM的特征,通过评估接近曲线,可以区分hBLM的区域是否被电穿孔。hBLM的基于SECM的电穿孔是通过使用参比电极和UME之间增加的电势差来进行的。取决于所施加的电势脉冲的持续时间以及取决于UME与hBLM表面之间的距离,实现了hBLM的不可逆或可逆电穿孔。数据表明SECM可成功用于hBLM的电穿孔和表征。取决于所施加的电势脉冲的持续时间以及取决于UME与hBLM表面之间的距离,实现了hBLM的不可逆或可逆电穿孔。数据表明SECM可成功用于hBLM的电穿孔和表征。取决于所施加的电势脉冲的持续时间以及取决于UME与hBLM表面之间的距离,实现了hBLM的不可逆或可逆电穿孔。数据表明SECM可成功用于hBLM的电穿孔和表征。

更新日期:2020-07-29
down
wechat
bug