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Cultivation of heterotrophic algae on enzymatically hydrolyzed municipal food waste
Algal Research ( IF 5.1 ) Pub Date : 2020-07-21 , DOI: 10.1016/j.algal.2020.101993
O. Haske-Cornelius , T. Vu , C. Schmiedhofer , R. Vielnascher , M. Dielacher , V. Sachs , M. Grasmug , S. Kromus , G.M. Guebitz

Municipal food waste is a unique source of biomass but with great variances in occurrence and properties. Economic, cultural and biophysical factors influence its composition. Therefore, investigation of novel applications requires experiments with real materials obtained from industrial processings, rather than tests with self-composed waste samples having a highly controlled composition. In this study, cultivation of the heterotrophic algae Chlorella sorokiniana on enzymatically hydrolyzed food waste was investigated. Municipal food waste of industrial origin hydrolyzed in a 1000-l stirred tank reactor led to an increase in glucose content from approximately 10 g l−1 to 50 g l−1 within 6 h. Chlorella sorokiniana grown on this medium exhibited an optical density of 32 corresponding to algae biomass concentration of approx. 9.5 g l−1 at a glucose concentration of 20 g l−1. The maximum biomass concentration was reached within four days under significant consumption of carbon and nitrogen. Approximately 30% of the carbon and 25% of the nitrogen were used, whereby glucose and acetic acid were the main carbon compounds consumed by C. sorokiniana in a diauxic manner. Other molecules like lactic acid were not metabolized, suggesting their conversion to biogas in a cascade approach. Nutrients present in the cultivation medium of C. sorokiniana are beneficial for subsequent anaerobic digestion. With a protein content of 37.8% and a lipid content of 26.4%, the composition of algae biomass was comparable to biomass grown on synthetic and other waste derived media.



中文翻译:

酶解市政食物垃圾的异养藻类培养

市政食物垃圾是生物质的独特来源,但其发生和性质差异很大。经济,文化和生物物理因素影响其组成。因此,对新应用的研究需要使用从工业加工中获得的真实材料进行实验,而不是使用具有高度受控成分的自组成废物样品进行测试。在这项研究中,研究了在酶解的食物垃圾上培养异养藻藻小球藻。在1000升搅拌釜反应器中水解的工业来源的市政食物垃圾导致葡萄糖含量在6小时内从大约10 g l -1增加到50 g l -1小球藻在这种培养基上生长的光密度为32,对应于藻类生物质的浓度约。9.5克升-1以20g l的葡萄糖浓度-1。在大量消耗碳和氮的情况下,四天内达到了最大生物量浓度。使用了大约30%的碳和25%的氮,其中葡萄糖和乙酸是C消耗的主要碳化合物。sorokiniana以双生的方式。其他分子(如乳酸)没有被代谢,表明它们以级联方式转化为沼气。C的培养基中存在营养素。索罗基亚纳对随后的厌氧消化有益。藻类生物质的蛋白质含量为37.8%,脂质含量为26.4%,与合成和其他废物衍生培养基上生长的生物质相当。

更新日期:2020-07-21
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