The aim of this study was to evaluate the effect of biofunctionalization with two TGF-β1 inhibitor peptides, P17 and P144, on osseointegration of CP-Ti dental implants. A total of 36 implants (VEGA, Klockner®) with 3.5 × 8 mm internal connection were used in this study, divided in three groups: (1) control group (n = 12), (2) implants which surfaces were biofunctionalized with P17 peptide inhibitor (n = 12), (3) implants with surfaces biofunctionalized by P144 peptide (n = 12). Three implants, one from each group, were inserted in both hemimandibles of 6 beagle dogs, 2 months after tooth extraction. Two animals were sacrificed at 2, 4 and 8 weeks post implant insertion, respectively. The samples were analyzed by Backscattering Scanning Electron Microscopy (BS-SEM) and histological analysis. Histomorphometric analysis of bone to implant contact (BIC), peri-implant bone fraction (BF) and interthread bone (IB) were carried out. Bone formation around implants measured by quantitative analysis, BS-SEM, was significantly higher in the P17-biofunctionalized implants, 4 and 8 weeks after the implantation. Histomorphometric analysis of BIC, BF and IB showed higher values in the P17-biofunctionalized group at initial stages of healing (2 weeks) and early osseointegration both at 4 and 8 weeks. For P144 biofunctionalized implants, the histomorphometric values obtained are also higher than control group. Accordingly, better results in the experimental groups were proven both by the quantitative and the qualitative analysis. Surface biofunctionalization with TGF-β1 inhibitor peptides, P17 and P144, resulted in better quantitative and qualitative parameters relative to implant osseointegration.

这项研究的目的是评估两种TGF-β1抑制剂肽P17和P144的生物功能化对CP-Ti牙科植入物骨整合的影响。本研究共使用36个具有3.5×8 mm内部连接的植入物（VEGA，Klockner®），分为三组：（1）对照组（n  = 12），（2）表面被P17生物功能化的植入物肽抑制剂（n  = 12），（3）具有P144肽生物功能化表面的植入物（n = 12）。拔牙后2个月，将3种植入物（每组1种）插入6只比格犬的半透明性。分别在植入植入物后的2、4和8周处死两只动物。通过背散射扫描电子显微镜（BS-SEM）和组织学分析对样品进行分析。进行了骨与种植体接触（BIC），种植体周围骨部分（BF）和螺纹间骨（IB）的组织形态分析。在植入后4周和8周，通过定量分析（BS-SEM）测量的植入物周围的骨形成明显高于P17生物功能化植入物。BIC，BF和IB的组织形态计量学分析显示，在康复的初始阶段（2周）和第4周和第8周的早期骨整合，P17生物功能化组的值更高。对于P144生物功能化植入物，获得的组织形态学值也高于对照组。因此，通过定量和定性分析证明了实验组中更好的结果。TGF-β1抑制剂肽P17和P144的表面生物功能化导致相对于植入物骨整合更好的定量和定性参数。