This study evaluated the effects of bioidentical nanostructured progesterone alone or in association with human chorionic gonadotropin (hCG) on the in vitro survival and development of preantral follicles (experiment 1) and oocyte maturation (experiment 2). Bioidentical hormones have a molecular structure identical with that of endogenous hormones; nanostructured substances refer to those reduced to a nanoscale. In experiment 1, fragments of goat ovarian tissue were cultured for 7 days in α-MEM + alone or supplemented with nanoprogesterone (MEM + + P4) or P4 and hCG (MEM + + P4 + hCG). In experiment 2, two mediums of oocyte in vitro maturation (IVM) were compared. Medium 1 consisted of TCM 199 + + LH, and medium 2 consisted of TCM 199 + with nanoprogesterone and hCG. The MEM + + P4 + hCG treatment showed the lowest percentage of follicular survival after 7 days of culture. MEM + + P4 and MEM + + P4 + hCG treatments showed higher percentage of follicular activation than MEM + . In experiment 2, there were no differences between mediums 1 and 2 for all endpoints evaluated. In conclusion, the addition of nanoprogesterone is advisable for in vitro culture of preantral follicles and oocyte maturation. However, the association of nanoprogesterone with hCG causes the cellular death of initial follicles but shows efficacy in IVM.

中文翻译：

---

生物相同纳米结构孕酮在体外培养窦前卵泡和卵母细胞成熟中的作用

本研究评估了生物相同纳米结构孕酮单独或与人绒毛膜促性腺激素 (hCG) 联合对体外存活和腔前卵泡发育（实验 1）和卵母细胞成熟（实验 2）的影响。生物同质激素具有与内源性激素相同的分子结构；纳米结构物质是指缩小到纳米级的物质。在实验 1 中，山羊卵巢组织片段在单独的 α-MEM + 或补充有纳米黄体酮 (MEM + + P4) 或 P4 和 hCG (MEM + + P4 + hCG) 中培养 7 天。在实验 2 中，比较了两种卵母细胞体外成熟 (IVM) 培养基。培养基 1 由 TCM 199 + + LH 组成，培养基 2 由 TCM 199 + 与纳米黄体酮和 hCG 组成。MEM + + P4 + hCG 处理显示培养 7 天后滤泡存活率最低。MEM + + P4 和 MEM + + P4 + hCG 处理显示出比 MEM + 更高的卵泡激活百分比。在实验 2 中，对于所有评估的终点，介质 1 和 2 之间没有差异。总之，对于腔前卵泡的体外培养和卵母细胞成熟，建议添加纳米孕酮。然而，纳米黄体酮与 hCG 的结合导致初始卵泡的细胞死亡，但在 IVM 中显示出功效。对于窦前卵泡的体外培养和卵母细胞成熟，建议添加纳米孕酮。然而，纳米黄体酮与 hCG 的结合导致初始卵泡的细胞死亡，但在 IVM 中显示出功效。对于窦前卵泡的体外培养和卵母细胞成熟，建议添加纳米孕酮。然而，纳米黄体酮与 hCG 的结合导致初始卵泡的细胞死亡，但在 IVM 中显示出功效。