Bioprocessing of pulp requires lignolytic as well as hemicellulolytic enzymes. The present study is the first report of a cocktail of laccase (L), xylanase (X), and mannanase (M), from a single bacterium for pulp biobleaching. A novel strain Bacillus tequilensis LXM 55 produced thermo-alkali stable L + X + M. On optimization higher enzyme yield (IUml⁻¹/fold increase) of laccase (396.35/24.16), xylanase (212.95/81.90) and mannanase (153.33/102.90) were achieved in the cocktail. Treatment of pulp with cocktail of enzymes led to 49.35% reduction in kappa number and considerable enhancement in the brightness (11.59%), whiteness (4.11%), and other pulp properties. Most importantly, no mediator system was required for the application of laccase. 40% less chlorine consumption was required to obtain the paper of the same quality as that of pulp treated without enzyme but with 100% chlorine. Therefore, this cocktail of enzymes is highly suitable for pulp biobleaching in the paper mill.

纸浆的生物加工需要木质素分解酶和半纤维素分解酶。本研究首次报道了来自单一细菌的用于纸浆生物漂白的漆酶 (L)、木聚糖酶 (X) 和甘露聚糖酶 (M) 混合物。一种新菌株龙舌兰芽孢杆菌LXM 55 产生热碱稳定的 L + X + M。优化更高的酶产量 (IUml ^-1在混合物中实现了漆酶 (396.35/24.16)、木聚糖酶 (212.95/81.90) 和甘露聚糖酶 (153.33/102.90) 的 / 倍增加。用酶混合物处理纸浆导致卡伯值降低 49.35%，亮度 (11.59%)、白度 (4.11%) 和其他纸浆特性显着提高。最重要的是，漆酶的应用不需要介体系统。要获得与未经酶处理但使用 100% 氯处理的纸浆质量相同的纸张，所需的氯消耗量减少 40%。因此，这种酶混合物非常适合造纸厂的纸浆生物漂白。