Abstract

Background

Short Tandem Repeats (STRs) are widely used in population and forensic genetic studies.

Aim

The objective of this study was to document the level and extent of genetic variation of the FBI Combined DNA Index System (CODIS) STR loci (D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, TH01, TPOX and CSF1PO) in 6 populations (British, Indian (Punjabis and Gujaratis), Pakistani, Bangladeshi and Sri Lankan) of the East Midlands (UK). There is a lack of genetic research on the migrant South Asian populations.

Subjects and methods

DNA samples (N = 603) were analysed for 13 autosomal forensic STR loci along with the amelogenin locus following standard protocols. Data were analysed for genetic variation and a range of forensic indices.

Results

All loci were polymorphic in all populations with a variable degree of variation. Average observed heterozygosity was highest in Bangladeshi (0.803) and lowest in Punjabi (0.761). FGA locus had the highest power of discrimination (PD) in most populations.

Conclusion

FGA locus was most polymorphic and discriminatory among migrant populations demonstrating it as the marker with the highest potential in forensic analyses. These results could be useful for population and forensic genomic studies.

中文翻译：

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基于CODIS法医STR基因座的英国本地人和东米德兰兹地区（英国）的五个南亚移民人口的遗传变异和分化。

摘要

背景

短串联重复序列（STRs）被广泛用于人群和法医遗传学研究。

目标

这项研究的目的是记录FBI组合DNA指标系统（CODIS）STR位点（D3S1358，vWA，FGA，D8S1179，D21S11，D18S51，D5S818，D13S317，D7S820，​​D16S539，TH01， TPOX和CSF1PO）分布在东米德兰兹地区（英国）的6个人口（英国，印度（旁遮普和古吉拉特人），巴基斯坦，孟加拉国和斯里兰卡）中。缺乏对南亚移民人口的遗传研究。

主题与方法

按照标准规程，对DNA样品（N = 603）中的13个常染色体法医学STR基因座以及釉原蛋白基因座进行了分析。分析数据的遗传变异和一系列取证指标。

结果

在所有种群中，所有基因座都是多态的，变异程度不同。平均观察到的杂合度在孟加拉国最高（0.803），在旁遮普邦最低（0.761）。在大多数人群中，FGA基因座具有最高的鉴别力（PD）。

结论

FGA位点在移民人群中具有最多的多态性和歧视性，表明它是法医分析中潜力最大的标记。这些结果可能对人口和法医基因组研究有用。