Abstract

Rosmarinic acid (RA) is an active constituent of Ocimum basilicum. It has been shown that hairy root production (measured as dry weight) improves when green basil (O. basilicum “Cinnamon”) is cultured under the light. In contrast, purple basil (O. basilicum “Purpurascens”) shows greater hairy root production when cultured under dark conditions. The level of gene expression was highest in hairy roots of green basil under dark conditions for up to 1 week. Transcript levels were highest in hairy roots of purple basil under both dark and light conditions after 2 weeks of culturing. After 3 weeks of culture under light conditions, green basil had accumulated 1.9-fold higher RA content than that of purple basil, which in turn was fivefold higher than that of the natural roots (42.86 µg/mg). Tyrosine aminotransferase showed a higher transcript level when compared to the other phenylpropanoid pathway genes (phenylalanine ammonia-lyase, cinnamate 4-hydroxylase, and coenzyme-A ligase) in both dark and light conditions and in all-time regimens. RA accumulation was higher in the cultured hairy roots of green basil than those of purple basil under both light and dark conditions.

摘要

迷迭香酸（RA）是巴西罗勒（Ocimum basilicum）的活性成分。研究表明，在光下培养绿色罗勒（O. basilicum “ Cinnamon”）时，毛根的产量（以干重衡量）会提高。相比之下，紫色罗勒（O. basilicum在黑暗条件下培养时，“ Purpurascens”显示出更大的毛根产生。在长达1周的黑暗条件下，绿色罗勒毛状根中的基因表达水平最高。培养2周后，紫罗勒毛状根在黑暗和明亮条件下的转录水平最高。在光照条件下培养3周后，绿色罗勒的RA含量比紫色罗勒高1.9倍，而紫色罗勒则比自然根（42.86 µg / mg）高5倍。与其他苯丙氨酸途径基因（苯丙氨酸氨裂合酶，肉桂酸4-羟化酶和辅酶-A连接酶）相比，酪氨酸氨基转移酶在黑暗和明亮条件下以及在所有治疗方案下均表现出更高的转录水平。