Objective

Following brain injury, the neurogenic niche provides a permissive cue for iatrogenesis rather than neurogenesis; reactive astrocytes play essential roles in orchestrating this process, markedly forming a glial scar around the area of damaged brain tissue. The objective of this study was to alter the neurogenic niche at the injured cortex and study its impact on neurogenesis.

Methods

We constructed a stromal cell-derived factor 1 (SDF-1) gradient matrix to attract reactive astrocytes to the glial scar core.

Results

SDF-1 reacted with the astrocytes in the injured site. By changing the neurogenic niche of the injured part of the brain after traumatic brain injury (TBI), SDF-1 downregulated thrombospondin 4 (Thbs4) promoting neuronal cell regeneration and playing a beneficial role in nerve function recovery after brain injury.

Discussion

The matrix we created in this study could attract and interact with reactive glial cells and, thus, we called it a glial pump. Using the glial pump, we identified a new mechanism of brain injury repair and neuronal regeneration after TBI, which relied on Thbs4 downregulation after the altered neurogenic niche promoted neuronal regeneration and functional recovery.

中文翻译：

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由神经源性利基变化引起的Thbs4的下调促进了脑外伤后的神经元再生。

目的

脑损伤后，神经源性利基为医源性而非神经发生提供了提示。反应性星形胶质细胞在协调这个过程中起着至关重要的作用，在受损的脑组织区域周围明显形成神经胶质瘢痕。这项研究的目的是改变受损皮层的神经源性利基，并研究其对神经发生的影响。

方法

我们构建了一个基质细胞衍生因子1（SDF-1）梯度矩阵，将反应性星形胶质细胞吸引到胶质瘢痕核心。

结果

SDF-1与受损部位的星形胶质细胞反应。通过改变外伤性脑损伤（TBI）后大脑受伤部位的神经源性利基，SDF-1下调血小板反应蛋白4（Thbs4）促进神经元细胞再生，并在脑损伤后神经功能恢复中发挥有益作用。

讨论区

我们在这项研究中创建的基质可以吸引反应性神经胶质细胞并与之相互作用，因此，我们将其称为神经胶质泵。使用神经胶质泵，我们确定了TBI后脑损伤修复和神经元再生的新机制，这依赖于改变的神经源性利基促进神经元再生和功能恢复后Thbs4的下调。