Abstract Reactive oxygen species including free radicals are responsible for noxious changes in living organisms which can be associated with many diseases for e.g. cardiovascular, neurodegenerative, and inflammatory diseases or cancer. In the struggle against free radicals the organism is supported by antioxidants. As they are often provided by nature, they can be considered safe. 5-Caffeoylquinic acid is one of main chlorogenic acids found in vegetables and fruits. The main objective of the presented article was monitoring the changes of 5-caffeoylquinic acid during the reaction with colorful radicals. The experiments were conducted at different molar ratios of radicals to antioxidant using liquid chromatography–mass spectrometry. Irrespective of the applied molar ratios, the obtained results proved that the antioxidant takes part in the reaction for three days. Additionally, during the radical neutralization the formation of semiquinones and/or quinones, methoxy adduct of caffeoylquinic acid, and quinones adduct with methanol was observed. Graphical Abstract

中文翻译：

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LC-MS监测5-咖啡酰奎宁酸与ABTS阳离子自由基反应过程中的变化

摘要 包括自由基在内的活性氧是造成生物体有害变化的原因，这些变化可能与许多疾病有关，例如心血管疾病、神经退行性疾病和炎症性疾病或癌症。在对抗自由基的斗争中，有机体得到抗氧化剂的支持。由于它们通常由大自然提供，因此可以被认为是安全的。5-咖啡酰奎宁酸是蔬菜和水果中的主要绿原酸之一。本文的主要目的是监测 5-咖啡酰奎宁酸与彩色自由基反应过程中的变化。使用液相色谱-质谱法在自由基与抗氧化剂的不同摩尔比下进行实验。无论应用的摩尔比如何，所得结果证明抗氧化剂参与反应三天。此外，在自由基中和过程中，观察到半醌和/或醌、咖啡酰奎宁酸的甲氧基加合物和醌与甲醇的加合物的形成。图形概要