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A GC-MS method validation for quantitative investigation of some chemical markers in Salvia hypargeia Fisch. & C.A. Mey. of Turkey: Enzyme inhibitory potential of ferruginol.
Journal of Food Biochemistry ( IF 4 ) Pub Date : 2020-07-19 , DOI: 10.1111/jfbc.13350
Derya Bakir 1 , Mehmet Akdeniz 1 , Abdulselam Ertas 2 , Mustafa A Yilmaz 3 , Ismail Yener 4 , Mehmet Firat 5 , Ufuk Kolak 6
Affiliation  

In the present study, a GC‐MS method used for quantitative screening of 26 compounds (sclareolide, sclareol, ferruginol, cryptanol, 6,7‐dehydroroyleanone, suginal, 9,10‐dihydro‐7,8‐dimethyl‐2‐(1‐methylethyl) phenanthren‐3‐ol, sugiol, inuroyleanone, 12‐demethylmulticauline, 7α‐hydroxy‐β‐sitosterol, stigmasterol, sitosterol, salvigenin, sinensetin, α‐amyrin, lupeol, lupenone, 3‐acetyl lupeol, 1α,21α‐dihydroxy‐2,3‐(1′1′‐dimethyl‐dioxymethylene) urs‐9(11),12‐dien, uvaol, betulin, pyxinol, lup‐(20),29‐ene‐2α‐hydroxy‐3β‐acetate, betulin 3β, 28β‐diacetate, 21α‐hydroxy,2α,3β‐diacetoxy urs‐9(11),12‐dien) specific to Turkish Salvia species was developed and validated. According to the GC–MS analysis results, Salvia hypargeia Fisch. & C.A. Mey. roots were found to be rich in ferruginol (30787.97 µg/g extract) and lupenone (23276.21 µg/g extract), and leaves in lupeol (20625.92 µg/g extract). Additionally, the essential oil and aroma contents of this species were identified by GC‐MS technique. According to the LC‐MS/MS results, especially S. hypargeia leaf extract was rich in rosmarinic acid (38035.7 µg/g extract) and isoquercitrin (4136.91 µg/g extract). Furthermore, anticholinesterase, antiurease, antityrosinase and antielastase inhibitory, antioxidant, cytotoxic activities of the ethanol extracts, essential oil, and major components of the species were evaluated. Antioxidant potentials of all extracts of this species were quite high in all studied antioxidant methods. Moreover, butyrylcholinesterase and elastase inhibitory capacities of ferruginol, the major component of S. hypargeia roots, were notable. For these reasons, this species has a high potential for food and pharmaceutical industries.

中文翻译:

定量分析丹参中某些化学标记物的GC-MS方法验证。和CA Mey。土耳其共和国:呋喃固醇的酶抑制潜力。

在本研究中,GC-MS方法用于定量筛查26种化合物(香紫苏内酯,香紫苏醇,铁氨新城,隐孢子醇,6,7-脱氢吡咯烷酮,Suginal,9,10-二氢-7,8-二甲基-2-(1甲基乙基)菲-3-醇,sugiol,inuroyleanone,12 demethylmulticauline,7 α羟基β谷甾醇,豆甾醇,谷甾醇,salvigenin,sinensetin,α -amyrin,羽扇豆醇,羽扇豆烯酮,3-乙酰基羽扇豆醇,1个α, 21 α -二羟基- 2,3-(1'1'二甲基二氧亚甲基)URS-9(11),12-二烯,uvaol,白桦脂醇,pyxinol,lup-(20),29烯-2- α -羟基-3 β -乙酸甲酯,桦木醇3 β,28 β -二乙酸根,21 α -羟基,2 α,3个β -diacetoxy URS-9(11),12-二烯)特定土耳其丹参物种被开发和验证。根据GC-MS分析结果,Salvia hypargeia Fisch。和CA Mey。根部富含铁氨固醇(30787.97 µg / g提取物)和羽扇豆酮(23276.21 µg / g提取物),叶中富含羽扇豆醇(20625.92 µg / g提取物)。此外,通过GC-MS技术鉴定了该物种的精油和香气含量。根据LC-MS / MS结果,尤其是hypergeia叶提取物富含迷迭香酸(38035.7 µg / g提取物)和异槲皮苷(4136.91 µg / g提取物)。此外,还评估了乙醇提取物,精油和该物种主要成分的抗胆碱酯酶,抗脲酶,抗酪氨酸酶和抗弹性蛋白酶的抑制作用,抗氧化剂,细胞毒性活性。在所有研究的抗氧化剂方法中,该物种所有提取物的抗氧化潜力都很高。此外,值得注意的是,S。hypargeia根的主要成分铁氨酚的丁酰胆碱酯酶和弹性蛋白酶抑制能力。由于这些原因,该物种在食品和制药行业具有很高的潜力。
更新日期:2020-09-14
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