As a traditional plant medicine in tropical areas, Swietenia macrophylla seeds are usually applied for some chronic diseases, including hypertension, diabetes, and so on. Few studies have been carried out to identify the effective elements in seed extract and their indications. In this study, we first investigated the functions of the swietenine, an extract from S. macrophylla seeds, using a model of myocardial hypertrophy induced by isoprenaline (ISO). At cellular level, H9c2 cell hypertrophy was also established through the treatment with ISO. The cardiac pathological remodeling was evaluated by echocardiography and histological analysis. Western blot and RT‐qPCR were used to detect the expression of possible hypertrophy‐promoting genes. Here, our results indicated that swietenine remarkably attenuated ISO‐induced myocardial hypertrophy in vivo and in vitro. Moreover, Akt phosphorylation, ANP and BNP mRNA expression were efficiently decreased. Based on these findings, we concluded that swietenine might be a promising anti‐hypertrophic agent against cardiac hypertrophy.

中文翻译：

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Swietenia 提取的 Swietenine 减轻异丙肾上腺素诱导的小鼠心肌肥厚

作为热带地区的传统植物药材，大叶猪肝种子通常用于治疗一些慢性病，包括高血压、糖尿病等。很少有研究确定种子提取物中的有效元素及其适应症。在这项研究中，我们首先使用异丙肾上腺素 (ISO) 诱导的心肌肥大模型研究了大叶海参种子提取物 swietenine 的功能。在细胞水平上，H9c2 细胞肥大也是通过 ISO 处理建立的。通过超声心动图和组织学分析评估心脏病理重塑。使用蛋白质印迹和 RT-qPCR 检测可能的肥大促进基因的表达。这里，我们的结果表明，在体内和体外，swietenine 显着减弱了 ISO 诱导的心肌肥大。此外，Akt 磷酸化、ANP 和 BNP mRNA 表达有效降低。基于这些发现，我们得出结论，甜菜碱可能是一种有前途的抗心肌肥厚的抗肥厚剂。