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CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda.
Developmental Biology ( IF 2.7 ) Pub Date : 2020-07-20 , DOI: 10.1016/j.ydbio.2020.07.001
Yi Gao 1 , Xiaoxi Zhang 2 , Xiaojun Zhang 1 , Jianbo Yuan 1 , Jianhai Xiang 3 , Fuhua Li 1
Affiliation  

The compound eye in crustaceans is a main eye type in the animal kingdom, knowledge about the mechanism to determine the development of compound eye is very limited. Paired box protein 6 (Pax6) is generally regarded as a master regulator for eye development. In the present study, a genome-based analysis of the Pax6 gene in the ridge tail white prawn Exopalaemon carinicauda was performed and two members of Pax6 homologs, named Ec-Eyeless (EcEy) and Ec-Twin of eyeless (EcToy) were identified. To understand the function of these two homologs of Pax6 gene in the prawn, the CRISPR/Cas9 genome editing technique was applied to generate EcEy and EcToy knock-out (KO) prawns and their phenotypes were analyzed. The surviving EcEy-KO embryos and larvae exhibited severe abnormal eye morphology, suggesting that EcEy is necessary for the compound eye development in prawn, while no mutant phenotype was found in EcToy-KO individuals. These findings highlighted the conservative role of Pax6 gene in the compound eye formation, and the functional differentiation between EcEy and EcToy gene may reveal a novel regulating mechanism of Pax6 on the compound eye development in the decapods. These data will provide important information for understanding the regulation mechanism for crustacean compound eye development.



中文翻译:

CRISPR / Cas9介导的突变表明Pax6对Decapoda Exopalaemon carinicauda中复眼的发育至关重要。

甲壳类动物中的复眼是动物界中的主要眼睛类型,关于决定复眼发育的机理的知识非常有限。配对盒蛋白6Pax6)通常被视为眼睛发育的主要调节剂。在本研究中,对ridge尾白虾Exopalaemon carinicauda中的Pax6基因进行了基于基因组的分析,并鉴定Pax6同源物的两个成员,名为Ec-EyelessEcEy)和Ec-Twin of EyelessEcToy)。了解Pax6这两个同源物的功能虾中的基因,应用CRISPR / Cas9基因组编辑技术生成EcEyEcToy敲除(KO)对虾,并对它们的表型进行分析。存活的EcEy -KO胚胎和幼虫表现出严重的异常眼睛形态,这表明EcEy是对虾复眼发育所必需的,而在EcToy -KO个体中未发现突变表型。这些发现强调了Pax6基因在复眼形成中的保守作用,并且EcEyEcToy基因之间的功能分化可能揭示了Pax6的新型调节机制。十足动物的复眼发育。这些数据将为理解甲壳动物复眼发育的调控机制提供重要信息。

更新日期:2020-07-20
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