Derived from polycyclic aromatic hydrocarbons (PAHs), oxygenated-PAHs (oxy-PAHs) may pose hazards to aquatic organisms, which remain largely unknown. Takifugu obscurus is an important anadromous fish species of high economic and ecological values. In the present study, T. obscurus was acutely exposed to 44.29 µg l⁻¹ 9,10-phenanthrenequione (9,10-PQ) for 96 h. Changes of antioxidant indices and metabolite profiles in plasma were compared between 9,10-PQ treatment and the control. The results showed that 9,10-PQ treatment significantly increased malondialdehyde (MDA) content during 6 to 96 h, increased superoxide dismutase (SOD) and catalase (CAT) activities at 6 h, but decreased them at 96 h. These results indicated that 9,10-PQ induced oxidative stress to fish. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) analysis revealed that four metabolic pathways were influenced in response to treatment with 9,10-PQ, including glycerophospholipid metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, purine metabolism and sulfur metabolism. These pathways are associated with antioxidant mechanisms, biosynthesis of neurotransmitters and innate immune functions. Thus, the as-obtained results confirmed that 9,10-PQ induced oxidative stress and raised concerns of neurotoxicity and immunotoxicity to fish. Overall, the present study posed a high environmental risk of oxy-PAHs to aquatic ecosystems.

氧化多环芳烃（PAH）衍生自多环芳烃（PAH），可能对水生生物造成危害，目前尚不清楚。暗纹（Takifugu obscurus）是重要的无鳍鱼类，具有很高的经济和生态价值。在本研究中，暗色丁香球菌急性暴露于44.29 µg l ^-19,10-菲基苯醌（9,10-PQ）96小时。比较了9,10-PQ处理与对照组之间血浆中抗氧化剂指数和代谢产物谱的变化。结果表明，9,10-PQ处理在6至96 h期间显着增加了丙二醛（MDA）含量，在6 h显着增加了超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性，但在96 h降低了它们。这些结果表明9,10-PQ诱导了鱼的氧化应激。超高效液相色谱-质谱（UPLC-MS）分析显示，响应9,10-PQ处理会影响四个代谢途径，包括甘油磷脂代谢，苯丙氨酸，酪氨酸和色氨酸生物合成，嘌呤代谢和硫代谢。这些途径与抗氧化机制有关，神经递质的生物合成和先天免疫功能。因此，所获得的结果证实9，10-PQ引起氧化应激并引起对鱼类的神经毒性和免疫毒性的关注。总体而言，本研究对水生生态系统造成了含氧多环芳烃的高环境风险。