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Naringin, a Natural Flavonoid, Modulates UVB Radiation-Induced DNA Damage and Photoaging by Modulating NER Repair and MMPS Expression in Mouse Embryonic Fibroblast Cells.
Journal of Environmental Pathology, Toxicology and Oncology ( IF 2.4 ) Pub Date : 2020-01-01 , DOI: 10.1615/jenvironpatholtoxicoloncol.2020031914
Roopa NilamberLal Das 1 , Agilan Balupillai 2 , Ernest David 2 , Murali Santhoshkumar 2 , Sridevi Muruhan 3
Affiliation  

We have proven that naringin, a phytonutrient, diminishes oxidative damage and inflammatory responses by modulating PPAR-γ expressions in ultraviolet-B radiation (UVB)-induced NIH-3T3 cells. However, the role of naringin against DNA damage, photoaging, and apoptosis in NIH-3T3 cells has yet to be studied, necessitating investigation. We show that Naringin pretreatment significantly reduces UVB-induced alkaline DNA damage and potentially modulates NER gene (XPC, TFIIH, XPE, ERCC1, and GAPDH) expression, thereby augmenting DNA repair. We determined experimentally that naringin pretreatment prevents UVB-induced nuclear fragmentation in NIH-3T3 cells, as well as altering UVB-induced apoptotic marker (Bax, BCl-2, Caspase-9, and Caspase-3) expression in them. In addition, naringin pretreatment inhibits UVB-stimulated matrix metalloproteinase (MMP-2, MMP-9 and MMP-13) expression in these 3T3 cells. Therefore, we report that naringin can effectively avert UVB-mediated DNA damage, photoaging, and apoptosis in NIH-3T3 cells.

中文翻译:

柚皮素,一种天然的类黄酮,通过调节NER修复和MMPS在小鼠胚胎成纤维细胞中的表达来调节UVB辐射诱导的DNA损伤和光老化。

我们已经证明,柚皮素是一种植物营养素,可通过调节紫外线B辐射(UVB)诱导的NIH-3T3细胞中的PPAR-γ表达来减少氧化损伤和炎症反应。但是,柚皮苷对NIH-3T3细胞中DNA损伤,光老化和细胞凋亡的作用尚待研究,因此有必要进行研究。我们显示,柚皮苷预处理可显着降低UVB诱导的碱性DNA损伤,并可能调节NER基因(XPC,TFIIH,XPE,ERCC1和GAPDH)的表达,从而增强DNA修复。我们通过实验确定,柚皮苷预处理可防止UVB诱导的NIH-3T3细胞核分裂,并改变其中的UVB诱导的凋亡标记(Bax,BCl-2,Caspase-9和Caspase-3)表达。此外,柚皮苷预处理还可以抑制UVB刺激的基质金属蛋白酶(MMP-2,MMP-9和MMP-13)在这些3T3细胞中的表达。因此,我们报道柚皮苷可以有效避免NIH-3T3细胞中UVB介导的DNA损伤,光老化和细胞凋亡。
更新日期:2020-01-01
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