. Present-day intraspecific diversity has largely been shaped by previous climatic events, but the spatial and temporal scales of differentiation processes in most species remain to be clarified. In Europe, the Pleistocene glacial cycles have generated population structures that remain especially evident in montane taxa. The intraspecific variation of the European subalpine Yellow-spotted Ringlet, Erebia manto (Denis & Schiffermüller, [1775]), shows a hierarchical, two-level structure that allows us to study intermediate stages of speciation. Morphologically, three subspecies clusters have been described in this butterfly: the manto, bubastis and vogesiaca type. An allozyme study previously revealed two genetic lineages within the manto type, and two within the vogesiaca type, but lacked bubastis representatives. To further our knowledge of the intraspecific structure of E. manto, we sampled all known and presumed intraspecific groups and sequenced 1,496 base pairs of the mitochondrial gene COI for 152 specimens from 15 localities. A median joining haplotype network, based on 40 parsimony informative sites, confirmed the four allozyme based lineages. The bubastis type was acknowledged as a fifth genetic lineage, replacing the manto type populations in the southern part of the western Alps, and separated from it by a well-known zoogeographic borderline. We discuss how the present-day distributions, genetic relationships and timing of the differentiations align.

中文翻译：

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黄色斑点的小环斑马球菌Manto（Denis＆Schiffermüller，[1775]）的种内结构，特别参考了bubastis组：形态，同工酶和mtDNA数据（鳞翅目、,虫，y科）的整合。

。今天的种内多样性在很大程度上是由先前的气候事件决定的，但是大多数物种中分化过程的时空尺度尚待阐明。在欧洲，更新世的冰川周期产生了种群结构，这在山地分类群中仍然尤为明显。欧洲亚高山黄斑小环，Erebia manto（Denis＆Schiffermüller，[1775]）的种内变异显示出一种分层的两级结构，使我们能够研究物种形成的中间阶段。从形态上讲，这只蝴蝶描述了三个亚种簇：曼陀，布巴斯蒂斯和伏格齐亚型。以前的同工酶研究揭示了manto型有两个遗传谱系，而vogesiaca型有两个遗传谱系，但是缺少bubastis代表。为了进一步了解曼氏大肠杆菌的种内结构，我们对所有已知和假定的种内组进行了采样，并对来自15个地区的152个标本的线粒体基因COI的1,496个碱基对进行了测序。基于40个简约信息位点的中位连接单倍型网络确认了四个基于同工酶的谱系。bubastis型被认为是第五种遗传谱系，取代了阿尔卑斯山西部南部的manto型种群，并通过众所周知的动物地理学界线与之分离。我们讨论了当今的分布，遗传关系和分化时间如何一致。基于40个简约信息位点的中位连接单倍型网络确认了四个基于同工酶的谱系。bubastis型被认为是第五种遗传谱系，取代了阿尔卑斯山西部南部的manto型种群，并通过众所周知的动物地理学界线与之分离。我们讨论了当今的分布，遗传关系和分化时间如何一致。基于40个简约信息位点的中位连接单倍型网络确认了四个基于同工酶的谱系。bubastis型被认为是第五种遗传谱系，取代了阿尔卑斯山西部南部的manto型种群，并通过众所周知的动物地理学界线与之分离。我们讨论了当今的分布，遗传关系和分化时间如何一致。