Bark beetles belonging to the genus Dryocoetes (Coleoptera, Curculionidae, Scolytinae) are known vectors of fungi, such as the pathogenic species Grosmannia dryocoetidis involved in alpine fir (Abies lasiocarpa) mortality. Associations between hardwood-infesting Dryocoetes species and fungi in Europe have received very little research attention. Ectosymbiotic fungi residing in Ceratocystiopsis and Leptographium (Ophiostomatales, Sordariomycetes, Ascomycota) were commonly detected in previous surveys of the Dryocoetes alni-associated mycobiome in Poland. The aim of this study was to accurately identify these isolates and to provide descriptions of the new species. The identification was conducted based on morphology and DNA sequence data for six loci (ITS1-5.8S, ITS2-28S, ACT, CAL, TUB2, and TEF1-α). This revealed two new species, described here as Ceratocystiopsis synnematasp. nov. and Leptographium alneumsp. nov. The host trees for the new species included Alnus incana and Populus tremula. Ceratocystiopsis synnemata can be distinguished from its closely related species, C. pallidobrunnea, based on conidia morphology and conidiophores that aggregate in loosely arranged synnemata. Leptographium alneum is closely related to Grosmannia crassivaginata and differs from this species in having a larger ascomatal neck, and the presence of larger club-shaped cells.

中文翻译：

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与来自波兰的树皮甲虫Dryocoetes alni有关的两种新的蛇口目（Sordariomycetes）。

属于旱生小球藻属（鞘翅目，Curculionidae，Scolytinae）的树皮甲虫是已知的真菌载体，例如与高山冷杉（Abies lasiocarpa）死亡有关的致病菌Grosmannia dryocoetidis。在欧洲，有硬木侵害的干线虫物种与真菌之间的联系很少受到研究关注。以前在波兰对与Dryocoetes alni相关的菌群进行调查时，通常发现了存在于囊藻和io中的共生真菌（Ophiostomatales，Sordariomycetes，Ascomycota）。这项研究的目的是准确识别这些分离株并提供新物种的描述。基于形态学和DNA序列数据对六个基因座（ITS1-5.8S，ITS2-28S，ACT，CAL，TUB2和TEF1-α）进行了鉴定。这揭示了两个新物种，这里描述为Ceratocystiopsis synnematasp。十一月 和L。十一月 新物种的寄主树包括included木（Alnus incana）和胡杨（Populus tremula）。根据分生孢子形态和分生孢子聚集在松散排列的分枝杆菌中，可以将其与紧密相关的物种C. pallidobrunnea区别开来。Leptographium alneum与Grosmannia crassivaginata密切相关，并且与该物种的不同之处在于其无子巢颈较大，并且存在较大的棍状细胞。基于分生孢子的形态和分生孢子聚集在松散排列的结膜中。Leptographium alneum与Grosmannia crassivaginata密切相关，并且与该物种的不同之处在于其无子巢颈较大，并且存在较大的棍状细胞。基于分生孢子的形态和分生孢子聚集在松散排列的结膜中。Leptographium alneum与Grosmannia crassivaginata密切相关，并且与该物种的不同之处在于其无虫眼颈部较大，并且存在较大的棍状细胞。