Background Permafrost, frozen ground cemented with ice, occupies about a quarter of the Earth’s hard surface and reaches up to 1000 metres depth. Due to constant subzero temperatures, permafrost represents a unique record of past epochs, whenever it comes to accumulated methane, oxygen isotope ratio or stored mummies of animals. Permafrost is also a unique environment where cryptobiotic stages of different microorganisms are trapped and stored alive for up to hundreds of thousands of years. Several protist strains and two giant protist viruses isolated from permafrost cores have been already described. New information In this paper, we describe a collection of 35 amoeboid protist strains isolated from the samples of Holocene and Pleistocene permanently frozen sediments. These samples are stored at −18°C in the Soil Cryology Lab, Pushchino, Russia and may be used for further studies and isolation attempts. The collection strains are maintained in liquid media and may be available upon request. The paper also presents a dataset which consists of a table describing the samples and their properties (termed "Sampling events") and a table describing the isolated strains (termed "Occurrences"). The dataset is publicly available through the GBIF portal.

中文翻译：

---

Frozen Zoo：包含活的原生生物及其病毒的永久冻土样本集合。

背景 永久冻土是用冰粘合的冻土，约占地球坚硬表面的四分之一，深度可达 1000 米。由于恒定的零度以下温度，永久冻土代表了过去时代的独特记录，无论是积累的甲烷、氧同位素比还是储存的动物木乃伊。永久冻土也是一个独特的环境，其中不同微生物的隐生阶段被捕获并存活长达数十万年。已经描述了从永久冻土核心分离的几种原生生物菌株和两种巨型原生生物病毒。新信息 在本文中，我们描述了从全新世和更新世永久冷冻沉积物样品中分离出的 35 种变形虫原生菌株的集合。这些样品储存在 -18°C 的土壤低温实验室，Pushchino，俄罗斯可能会被用于进一步研究和隔离尝试。收集菌株保存在液体培养基中，可根据要求提供。该论文还提供了一个数据集，该数据集由一个描述样本及其属性的表（称为“采样事件”）和一个描述分离菌株的表（称为“发生”）组成。该数据集可通过 GBIF 门户公开获得。