ABSTRACT

Objectives

There is conflicting evidence on the effect of physical exercise on migraine development. Present study investigated the impact of treadmill exercise on migraine – associated symptoms and changes in calcitonin gene-related peptide (CGRP) expression in rats with and without maternal deprivation stress (MD).

Methods

Two days after birth, the male Wistar pups were randomly divided into four groups (n = 6) as follows: intact, exercise, MD, and MD plus exercise. The animals in the MD groups were separated from their dams 4 h per day for 2 weeks. At 8 weeks of age, the rats were exercised on a motor-driven treadmill for 4 weeks. Then, nitroglycerin (NTG) (5 mg/kg/IP) was used to induce migraine and pain-related symptoms were recorded for 90 min. NTG-related thermal hyperalgesia was measured by tail flick and hot plate methods. Finally, immunofluorescence staining of CGRP in trigeminal subnucleus caudalis (Vc) was performed.

Results

NTG – produced a significant headache symptoms and thermal hypersensitivity, which were aggravated following physical exercise in stressed or unstressed groups. Besides, NTG administration increased CGRP expression in the Vc of rats. Such effect was overpowered by treadmill running only in rats exposed to MD stress.

Conclusion

These findings highlight the worsening effects of treadmill exercise for migraine in rats with and without MD stress. However, inflammatory response can further exacerbate in stressed rats.

中文翻译：

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体育锻炼增强了对与应激大鼠三叉神经尾核中 CGRP 上调相关的偏头痛的易感性。

摘要

目标

关于体育锻炼对偏头痛发展的影响存在相互矛盾的证据。本研究调查了跑步机运动对偏头痛相关症状和降钙素基因相关肽 (CGRP) 表达变化的影响，在有和没有母体剥夺压力 (MD) 的大鼠中。

方法

出生两天后，雄性 Wistar 幼崽被随机分为四组 (n = 6)，如下所示：完整、运动、MD 和 MD 加运动。MD 组中的动物每天与它们的母猪分开 4 小时，持续 2 周。在 8 周龄时，大鼠在电动跑步机上锻炼 4 周。然后，使用硝酸甘油 (NTG) (5 mg/kg/IP) 诱发偏头痛并记录疼痛相关症状 90 分钟。NTG 相关的热痛觉过敏是通过甩尾和热板方法测量的。最后，在三叉神经尾核 (Vc) 中进行 CGRP 的免疫荧光染色。

结果

NTG – 产生显着的头痛症状和热过敏，在有压力或无压力的人群中进行体育锻炼后会加重。此外，NTG 给药增加了大鼠 Vc 中 CGRP 的表达。这种效果被仅在暴露于 MD 压力的大鼠中运行的跑步机所压制。

结论

这些发现强调了跑步机运动对有和没有 MD 压力的大鼠偏头痛的恶化影响。然而，应激大鼠的炎症反应会进一步加剧。