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Screening of Siderophore-Producing PGPRs Isolated from Groundnut (Arachis hypogaea L.) Rhizosphere and Their Influence on Iron Release in Soil
Communications in Soil Science and Plant Analysis ( IF 1.8 ) Pub Date : 2020-07-03 , DOI: 10.1080/00103624.2020.1791159
Sair Sarwar 1 , Abdul Khaliq 2 , Munazza Yousra 1 , Tariq Sultan 1 , Nazir Ahmad 3 , Muhammad Zameer Khan 1
Affiliation  

ABSTRACT Iron is indispensable for the growth and metabolism of all living organisms but its availability in soil is limited due to very low solubility of dominant ferric iron (Fe3+). Plant growth-promoting rhizobacteria (PGPRs) have the ability to synthesize low molecular weight siderophores having high affinity for iron. Considering the siderophore-producing ability of PGPRs, a study was designed to isolate potential siderophore-producing bacteria from the rhizosphere of groundnut grown in the Potohar region. A total of 120 (MGS-1 to MGS-120) different bacterial isolates were obtained and screened for siderophore production. Under the quantitative estimation of siderophores, the maximum (73%) siderophore production was observed in MGS-14 followed by MGS-11 (69%) and MGS-91 (68%). Furthermore, these isolates were used for the incubation study which was conducted to evaluate the effect of SPB isolates on iron release in the soil. The soil was incubated at 28°C for 96 days and field capacity was maintained with distilled water from time to time. The results of the study revealed that the three SPB isolates (MGS-11, MGS-14, and MGS-91) which produced maximum siderophore units (>60% SU) showed an increase in iron release and 82%, 71%, and 69% over control on 32nd day of incubation, respectively. Moreover, the efficient bacterial isolates were characterized through 16S rRNA gene sequence analysis. The identified bacterial isolates were Bacillus subtilis, Bacillus halotolerans, and Bacillus safensis.

中文翻译:

花生 (Arachis hypogaea L.) 根际分离的产铁素 PGPR 的筛选及其对土壤铁释放的影响

摘要 铁对于所有生物的生长和代谢都是必不可少的,但由于占优势的三价铁 (Fe3+) 的溶解度非常低,因此其在土壤中的可用性受到限制。植物生长促进根际细菌 (PGPR) 能够合成对铁具有高亲和力的低分子量铁载体。考虑到 PGPR 产生铁载体的能力,设计了一项研究,以从 Potohar 地区种植的花生根际中分离出潜在的产生铁载体的细菌。总共获得了 120 个(MGS-1 到 MGS-120)不同的细菌分离株,并筛选了铁载体生产。根据铁载体的定量估计,在 MGS-14 中观察到最大 (73%) 的铁载体产量,其次是 MGS-11 (69%) 和 MGS-91 (68%)。此外,这些分离株用于培养研究,该研究旨在评估 SPB 分离株对土壤中铁释放的影响。土壤在 28°C 下培养 96 天,并不时用蒸馏水保持田间持水量。研究结果显示,产生最大铁载体单位 (>60% SU) 的三种 SPB 分离株(MGS-11、MGS-14 和 MGS-91)显示铁释放增加,82%、71% 和在孵化第 32 天分别超过对照 69%。此外,通过16S rRNA基因序列分析表征了有效的细菌分离物。鉴定出的细菌分离株是枯草芽孢杆菌、耐盐芽孢杆菌和安全芽孢杆菌。土壤在 28°C 下培养 96 天,并不时用蒸馏水保持田间持水量。研究结果显示,产生最大铁载体单位 (>60% SU) 的三种 SPB 分离株(MGS-11、MGS-14 和 MGS-91)显示铁释放增加,82%、71% 和在孵化第 32 天分别超过对照 69%。此外,通过16S rRNA基因序列分析表征了有效的细菌分离物。鉴定出的细菌分离株是枯草芽孢杆菌、耐盐芽孢杆菌和安全芽孢杆菌。土壤在 28°C 下培养 96 天,并不时用蒸馏水保持田间持水量。研究结果表明,产生最大铁载体单位 (>60% SU) 的三种 SPB 分离株(MGS-11、MGS-14 和 MGS-91)显示铁释放增加,82%、71% 和在孵化第 32 天分别超过对照 69%。此外,通过16S rRNA基因序列分析表征了有效的细菌分离物。鉴定出的细菌分离株是枯草芽孢杆菌、耐盐芽孢杆菌和安全芽孢杆菌。在孵化第 32 天分别比对照高 69%。此外,通过16S rRNA基因序列分析表征了有效的细菌分离物。鉴定出的细菌分离株是枯草芽孢杆菌、耐盐芽孢杆菌和安全芽孢杆菌。在孵化第 32 天分别比对照高 69%。此外,通过16S rRNA基因序列分析表征了有效的细菌分离物。鉴定出的细菌分离株是枯草芽孢杆菌、耐盐芽孢杆菌和安全芽孢杆菌。
更新日期:2020-07-03
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