This study combines ultrasound-assisted extraction and vortex-assisted dispersive micro-solid phase extraction using an ionic imprinted polymer as a selective sorbent for rapid isolation and pre-concentration of inorganic arsenic species (As(III) and As(V)) in extracts from rice samples prior to their determination by high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry. All factors affecting the ultrasound assisted extraction of the species from rice (ultrasound amplitude, sonication time and sonication mode) and their selective pre-concentration by ionic imprinted polymer-based vortex-assisted dispersive micro-solid phase extraction (sorbent amount, extract pH, vortex extraction time and speed, eluting solution and vortex elution time and speed) were optimized. The analytical performance of the procedure was studied at optimum conditions: ultrasound continuous sonication at 40% amplitude for 2.0 min using 1:1 methanol/ultrapure as an extractant, 50 mg of sorbent, extract pH at 8.0, vortex loading at 1000 rpm for 1.0 min, and elution with ultrapure water by vortexing at 1000 rpm for 1.0 min, pre-concentration procedure which leads to a pre-concentration factor of 10. The limits of detection obtained for As (III) and As (V) were 0.20 and 0.41 μg kg⁻¹, respectively, and were well below the maximum levels established by the European Union in rice and rice containing products. The method was found to be precise (intraday and interday relative standard deviations ≤ 11%) and selective. The accuracy was confirmed by analysing the ERM-BC211 (rice, As species) certified reference material, and the method was successfully applied to commercial rice samples.

这项研究结合了超声辅助萃取和涡流辅助分散微固相萃取，其中使用离子印迹聚合物作为选择性吸附剂，可快速分离和预浓缩萃取物中的无机砷（As（III）和As（V））。高效液相色谱-电感耦合等离子体质谱法测定大米样品之前，先从大米样品中提取。超声波影响从稻米中提取物种的所有因素（超声振幅，超声处理时间和超声处理模式）以及通过离子印迹聚合物基涡旋辅助分散微固相萃取（吸附剂量，萃取液pH，优化了涡旋提取时间和速度，洗脱液和涡旋洗脱时间和速度。^-1，分别远低于欧盟确定的大米和含大米产品的最高含量。发现该方法是精确的（日内和日间相对标准偏差≤11％）且具有选择性。通过分析ERM-BC211（大米，砷物种）认证的参考物质，可以确认其准确性，该方法已成功应用于商业大米样品。