Transcriptional reinduction memory is a phenomenon whereby cells “remember” their transcriptional response to a previous stimulus such that subsequent encounters with the same stimulus can result in altered gene expression kinetics. Chromatin structure is thought to play a role in certain transcriptional memory mechanisms, leading to questions as to whether and how memory can be actively maintained and inherited to progeny through cell division. Here we summarize efforts towards dissecting chromatin-based transcriptional memory inheritance of GAL genes in Saccharomyces cerevisiae. We focus on methods and analyses of GAL (as well as MAL and INO) memory in single cells and discuss the challenges in unraveling the underlying mechanisms in yeast and higher eukaryotes.

转录还原记忆是一种现象，细胞通过这种现象可以“记住”它们对先前刺激的转录反应，从而使随后遇到的相同刺激会导致基因表达动力学改变。染色质结构被认为在某些转录记忆机制中起作用，从而引发了关于是否以及如何通过细胞分裂主动维持记忆并将其遗传给子代的疑问。在这里，我们总结了剖析酿酒酵母中GAL基因基于染色质的转录记忆遗传的努力。我们专注于GAL（以及MAL和INO）的方法和分析）记忆在单个细胞中，并讨论了揭示酵母和高等真核生物中潜在机制的挑战。