Dietary lysophospholipids would influence milk composition of sows, thus positively affect intestinal health of offspring. The objective of this study was to determine effects of dietary lysophospholipids fed to lactating sows on performance, milk characteristics, gut health, and gut associated microbiome of offspring. Sixty pregnant sows were allotted to 2 treatments in a randomized complete block design with parity and BW as blocks on d 110 of gestation. Treatments were CON (no added lysophospholipids) and LPL (0.05% lysophospholipids; Lipidol-Ultra, Pathway Intermediates, Shrewsbury, UK). Sows were fed 2 kg/d from d 110 of gestation until farrowing and ad libitum after farrowing. Diets were formulated to meet nutrient requirements suggested by NRC (2012). Colostrum and milk samples from 12 sows per treatment were collected to measure nutrients and immunoglobulins on d 1 and d 18 of lactation, respectively. Twelve piglets per treatment (1 piglet per litter) were euthanized on d 18 to collect tissues to measure tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), malondialdehyde, protein carbonyl, IgA, histomorphology, crypt cell proliferation rate, and microbiota in the jejunum and colon. Data were analyzed using the MIXED procedure of SAS and the mortality was analyzed using GLIMMIX procedure of SAS. There was no difference in sow BW, parity, and litter size between treatments on d 0 of lactation. Sows fed LPL had increased (P < 0.05) litter BW gain (53.9 vs. 59.4 kg) and decreased piglet mortality (13.9 vs. 10.6%) on d 18 of lactation. Sows fed LPL had increased (P < 0.05) omega-6:omega-3 (22.1 vs. 23.7) and unsaturated:saturated (1.4 vs. 1.6) fatty acids ratios with increased oleic acid (29.1 vs. 31.4%) and tended to have increased (P = 0.092) IgG (1.14 vs. 1.94 g/L) and linoleic acid (17.7 vs. 18.7%) in the milk on d 18 of lactation. Piglets from sows fed LPL had increased (P < 0.05) IL-8 (184 vs. 245 pg/mg) and crypt cell proliferation rate (39.4 vs. 40.9%) and tended to have increased (P = 0.095) Firmicutes:Bacteroidetes ratio (1.0 vs. 3.5) in the jejunum. In conclusion, sows fed with lysophospholipids had milk with increased IgG, oleic acids, and linoleic acids without changes in BW and backfat during lactation. These changes could contribute to improved survivability and intestinal health of piglets by increasing IL-8 concentration, enhancing balance among gut associated microbiome, and increasing enterocyte proliferation in the jejunum.

中文翻译：

---

哺乳期饮食中的溶血磷脂对母猪生产性能，牛奶成分，肠道健康以及后代肠道相关微生物组的补充作用。

饮食中的溶血磷脂会影响母猪的乳汁成分，从而对后代的肠道健康产生积极影响。这项研究的目的是确定饲喂泌乳母猪的饮食溶血磷脂对后代生产性能，牛奶特性，肠道健康和肠道相关微生物组的影响。在妊娠第110天，将60只妊娠母猪分配给2种处理，采用随机完整区组设计，以胎次和体重作为区组。治疗为CON（不添加溶血磷脂）和LPL（0.05％溶血磷脂； Lipidol-Ultra，Pathway Intermediates，英国什鲁斯伯里）。从妊娠第110天至分娩开始以2kg / d的速度饲喂母猪，分娩后自由采食。制定了满足NRC（2012）建议的营养要求的饮食。收集每次处理12头母猪的初乳和牛奶样品，分别在哺乳第1天和第18天测量营养和免疫球蛋白。在第18天对每处理12头小猪（每窝1头小猪）实施安乐死，收集组织以测量肿瘤坏死因子-α（TNF-α），白介素8（IL-8），丙二醛，羰基蛋白，IgA，组织形态学，隐窝细胞增殖率以及空肠和结肠中的微生物群。使用SAS的MIXED程序分析数据，并使用SAS的GLIMMIX程序分析死亡率。泌乳第0天的处理之间母猪体重，胎次和产仔数没有差异。饲喂LPL的母猪增加了（在第18天对每处理12头小猪（每窝1头小猪）实施安乐死，收集组织以测量肿瘤坏死因子-α（TNF-α），白介素8（IL-8），丙二醛，羰基蛋白，IgA，组织形态学，隐窝细胞增殖率以及空肠和结肠中的微生物群。使用SAS的MIXED程序分析数据，并使用SAS的GLIMMIX程序分析死亡率。泌乳第0天的处理之间母猪体重，胎次和产仔数没有差异。饲喂LPL的母猪增加了（在第18天对每处理12头小猪（每窝1头小猪）实施安乐死，收集组织以测量肿瘤坏死因子-α（TNF-α），白介素8（IL-8），丙二醛，羰基蛋白，IgA，组织形态学，隐窝细胞增殖率以及空肠和结肠中的微生物群。使用SAS的MIXED程序分析数据，并使用SAS的GLIMMIX程序分析死亡率。泌乳第0天的处理之间母猪体重，胎次和产仔数没有差异。饲喂LPL的母猪增加了（哺乳期d 0的处理之间的胎次和产仔数。饲喂LPL的母猪增加了（哺乳期d 0的处理之间的胎次和产仔数。饲喂LPL的母猪增加了（P <0.05）泌乳第18天增加体重（53.9 vs. 59.4 kg）和降低仔猪死亡率（13.9 vs. 10.6％）。饲喂LPL的母猪的omega-6：omega-3（22.1 vs. 23.7）和不饱和脂肪酸：饱和脂肪酸（1.4 vs. 1.6）的脂肪酸比例增加（29.1 vs. 31.4％），并且倾向于增加（P <0.05）哺乳第18天时牛奶中的IgG（1.14 vs. 1.94 g / L）和亚油酸（17.7 vs. 18.7％）增加（P = 0.092）。用LPL喂养的母猪的仔猪IL-8含量升高（P < 0.05）（184 vs. 245 pg / mg），隐窝细胞增殖率升高（39.4 vs. 40.9％），并有升高的趋势（P =0.095）空肠中的硬菌：拟杆菌比率（1.0对3.5）。总之，饲喂溶血磷脂的母猪的乳汁中的IgG，油酸和亚油酸增加，而泌乳过程中的体重和背脂却没有变化。这些变化可通过增加IL-8浓度，增强肠道相关微生物组之间的平衡以及增加空肠中肠细胞的增殖来改善仔猪的生存能力和肠道健康。