Introduction

Mini-invasive therapies based on autologous non-activated Platelet-Rich Plasma (ANA-PRP), Low-Level Laser Therapy (LLL-T), and Micro-Needling Technique (MN-T) used in combining for hair re-growth need to be standardized.

Objectives

The work aims to show in vivo outcomes resulted from retrospective case-series study in which ANA-PRP + MN-T + LLL-T were used in combined in patients affected by Androgenic alopecia.

Methods

23 patients were treated, of which 13 males were classified in stage I–V by the Norwood–Hamilton scale, and 10 females were classified in stage I–III by the Ludwig scale. Assessment of hair re-growth was evaluated with photography, physician’s and patient’s global assessment scale, and standardized phototrichograms during a follow-up: T0 – baseline, T1 – 12 weeks, T2 – 23 weeks, T3 – 44 weeks, T4 – 58 weeks.

Results

Interesting outcomes represented by a hair density increase of 81 ± 5 hairs/cm² and 57 ± 7 hairs/cm² respectively at T1 and T2 compared with baseline (173 ± 5 hairs/cm² at T1 and 149 ± 9 hairs/cm² at T2 versus 92 ± 2 hairs/cm² at baseline) were observed using computerized trichograms.

Expert Opinion

The main limitation in the autologous regenerative therapies and biotechnologies in hair-regrowth is the extreme variability of PRP products used, in the absence of standardized protocols and widely shared. Appropriate PRP preparations have to be pick after carefully thinking about their bio-molecular specifications and intended indications for use in patients. This approach will aid in matching the optimal PRP product to specific patient factors, leading to improved outcomes and the elucidation of the cost-effectiveness of this treatment.

The combined use of biotechnologies as the association of PRP with micro-needling and low-level laser therapy may improve the results in terms of hair count and hair density compared with those obtained by alone PRP. All the procedures must be performed in the full respect of international and local rules.

Conclusions

The effect of the combined use of MN-T, LLL-T, and ANA-PRP has been demonstrated.

中文翻译：

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一项随机双盲回顾性研究：微针技术，低水平激光疗法和自体非活化富血小板血浆的组合使用可改善雄激素性脱发患者的头发再生长。

介绍

基于自发性非活化富血小板血浆（ANA-PRP），低水平激光疗法（LLL-T）和微针技术（MN-T）的微创疗法，用于结合头发再生长的需求标准化。

目标

这项工作旨在显示回顾性病例系列研究的体内结果，该研究在患有雄激素性脱发的患者中联合使用ANA-PRP + MN-T + LLL-T。

方法

对23例患者进行了治疗，其中13例男性通过Norwood–Hamilton评分被划分为IV期，10例女性通过Ludwig评分被划分为I–III期。在随访过程中，通过摄影，医师和患者的整体评估量表以及标准化的照像照片对头发的生长进行评估：T0 –基线，T1 – 12周，T2 – 23周，T3 – 44周，T4 – 58周。

结果

有趣的结果是，与基线相比，T1和T2的头发密度分别增加了81±5毛/ cm ²和57±7毛/ cm ²（T1和173±5毛/ cm ²以及149±9毛/ cm ²在T2时，相对于基线时的92±2毛/ cm ²，使用计算机三角图观察到了。

专家意见

自发再生疗法和生物技术在头发再生方面的主要局限性在于所使用的PRP产品存在极大的变异性，缺乏标准化的协议并且没有广泛共享。在仔细考虑了它们的生物分子规格和预期在患者中使用的适应症后，必须选择合适的PRP制剂。这种方法将有助于使最佳的PRP产品与特定的患者因素相匹配，从而改善结果并阐明该治疗的成本效益。

与单独使用PRP所获得的结果相比，将生物技术与PRP与微针和低水平激光疗法相结合的组合使用可以改善结果，无论是毛发计数还是毛发密度。所有程序都必须在充分遵守国际和本地规则的情况下执行。

结论

MN-T，LLL-T和ANA-PRP结合使用的效果已得到证明。