Pure carbon sources, especially carbohydrates which are raw materials deriving from agro‐industrial processes, are often used for small‐scale single‐cell oil production by fermentation. The aim of this study was to investigate the effects of different pure carbon sources on cell growth, lipid accumulation, and γ‐linolenic acid (GLA) production by the filamentous fungus Mortierella isabellina DSM 1414 (Deutsche Sammlung von Mikroorganismen). The sugars utilized in this study are found extensively and abundantly in nature, especially in food raw materials and, in consequence, in agro‐food industry wastes or surpluses. Thus, the potential of many waste materials containing these sugars to be used in the production of single‐cell oil by fermentation could also be evaluated. The effects of the sugars utilized on cell growth, biomass production, and lipid production were investigated. Fatty acids were also analysed in the lipids produced at the end of the fermentations. Results showed that the maximum biomass production was 10.80 g/L in lactose‐based media, while the maximum oil production was 5.44 g/L in maltose‐based media. Oleic (20.42%–42.94%), palmitic (14.96%–22.19%), and stearic (9.00%–26.92%) acids were the major fatty acids along with linoleic acid (11.35%–18.67%) and GLA (3.56%–8.04%). The production of GLA as the target fatty acid was remarkable. This study indicates that agro‐industrial waste including most of the sugars utilized (except for arabinose and sucrose with lipid production of 0.81 and 0.28 g/L, respectively) can be employed for production of single‐cell oil by M. isabellina DSM 1414 which contains a high amount of GLA.

中文翻译：

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Mortierella isabellina DSM 1414 使用不同的糖作为碳源生产单细胞油。

纯碳源，尤其是源自农业工业过程的原材料碳水化合物，通常用于通过发酵进行小规模单细胞油生产。本研究的目的是研究不同纯碳源对丝状真菌Mortierella isabellina 的细胞生长、脂质积累和 γ-亚麻酸 (GLA) 产生的影响。DSM 1414 (Deutsche Sammlung von Mikroorganismen)。本研究中使用的糖在自然界中广泛而丰富，特别是在食品原料中，因此，在农业食品工业的废物或剩余物中。因此，还可以评估许多含有这些糖的废料用于发酵生产单细胞油的潜力。研究了所利用的糖对细胞生长、生物质产生和脂质产生的影响。还在发酵结束时产生的脂质中分析了脂肪酸。结果表明，基于乳糖的培养基中生物量的最大产量为 10.80 g/L，而麦芽糖培养基中的最大油产量为 5.44 g/L。油酸 (20.42%–42.94%)、棕榈酸 (14.96%–22.19%) 和硬脂酸 (9.00%–26)。92%) 酸是主要的脂肪酸，还有亚油酸 (11.35%–18.67%) 和 GLA (3.56%–8.04%)。作为目标脂肪酸的 GLA 的产生是显着的。该研究表明，包括大部分所利用糖类的农业工业废物（阿拉伯糖和蔗糖除外，其脂质产量分别为 0.81 和 0.28 g/L）可用于 M. isabellina DSM 1414 生产单细胞油。含有大量的 GLA。