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RNA interference of eclosion hormone gene reveals its roles in the control of ecdysis behavior in Heortia vitessoides Moore.
Archives of Insect Biochemistry and Physiology ( IF 2.2 ) Pub Date : 2020-07-18 , DOI: 10.1002/arch.21726 Zhixing Li 1 , Qingya Ye 1 , Zihao Lyu 1 , Tong Lin 1
Archives of Insect Biochemistry and Physiology ( IF 2.2 ) Pub Date : 2020-07-18 , DOI: 10.1002/arch.21726 Zhixing Li 1 , Qingya Ye 1 , Zihao Lyu 1 , Tong Lin 1
Affiliation
Eclosion hormone (EH) is an important neuropeptide that regulates growth and development. This study predicted the EH gene (HvEH) of Heortia vitessoides Moore (Lepidoptera: Crambidae) from the transcriptome database and its expression patterns were determined using quantitative real‐time polymerase chain reaction. HvEH was expressed in all developmental stages and especially in the head area. RNA interference‐mediated silencing of HvEH (2 μg/individual) with double‐stranded HvEH RNA (dsHvEH) was achieved within 48 hr. Abnormal phenotypes appeared in the pupa and adult stages. dsHvEH injection suppressed pupation and eclosion rates. HvEH expression increased upon treatment with 20‐hydroxyecdysone but decreased at extreme temperatures. These results suggest that HvEH plays an essential role in ecdysis and wing formation in H. vitessoides.
中文翻译:
提取激素基因的RNA干扰揭示了它在控制Heortia vitessoides Moore中的蜕皮行为中的作用。
促凝激素(EH)是调节生长和发育的重要神经肽。这项研究从转录组数据库中预测了Heortia vitessoides Moore(鳞翅目:Crambidae)的EH基因(HvEH),并使用定量实时聚合酶链反应确定了其表达模式。HvEH在所有发育阶段都表达,尤其是在头部区域。在48小时内,用双链HvEH RNA(ds HvEH)实现了RNA干扰介导的HvEH(2μg/个体)沉默。the和成年阶段出现异常表型。ds HvEH注射可抑制化脓和e出率。病毒性肝炎在用20-羟基蜕皮酮治疗后,其表达增加,但在极端温度下降低。这些结果表明,HvEH在H的蜕皮和翅膀形成中起着至关重要的作用。维特索德。
更新日期:2020-07-18
中文翻译:
提取激素基因的RNA干扰揭示了它在控制Heortia vitessoides Moore中的蜕皮行为中的作用。
促凝激素(EH)是调节生长和发育的重要神经肽。这项研究从转录组数据库中预测了Heortia vitessoides Moore(鳞翅目:Crambidae)的EH基因(HvEH),并使用定量实时聚合酶链反应确定了其表达模式。HvEH在所有发育阶段都表达,尤其是在头部区域。在48小时内,用双链HvEH RNA(ds HvEH)实现了RNA干扰介导的HvEH(2μg/个体)沉默。the和成年阶段出现异常表型。ds HvEH注射可抑制化脓和e出率。病毒性肝炎在用20-羟基蜕皮酮治疗后,其表达增加,但在极端温度下降低。这些结果表明,HvEH在H的蜕皮和翅膀形成中起着至关重要的作用。维特索德。