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Gene expression profile of adhesion and extracellular matrix molecules during early stages of skeletal muscle regeneration.
Journal of Cellular and Molecular Medicine ( IF 5.3 ) Pub Date : 2020-07-18 , DOI: 10.1111/jcmm.15624
Laura C Ceafalan 1, 2 , Maria Dobre 3 , Elena Milanesi 3, 4 , Andrei M Niculae 2 , Emilia Manole 1 , Mihaela Gherghiceanu 2, 5 , Mihail E Hinescu 1, 2
Affiliation  

Skeletal muscle regeneration implies the coordination of myogenesis with the recruitment of myeloid cells and extracellular matrix (ECM) remodelling. Currently, there are no specific biomarkers to diagnose the severity and prognosis of muscle lesions. In order to investigate the gene expression profile of extracellular matrix and adhesion molecules, as premises of homo‐ or heterocellular cooperation and milestones for skeletal muscle regeneration, we performed a gene expression analysis for genes involved in cellular cooperation, migration and ECM remodelling in a mouse model of acute crush injury. The results obtained at two early time‐points post‐injury were compared to a GSE5413 data set from two other trauma models. Third day post‐injury, when inflammatory cells invaded, genes associated with cell‐matrix interactions and migration were up‐regulated. After day 5, as myoblast migration and differentiation started, genes for basement membrane constituents were found down‐regulated, whereas genes for ECM molecules, macrophage, myoblast adhesion, and migration receptors were up‐regulated. However, the profile and the induction time varied according to the experimental model, with only few genes being constantly up‐regulated. Gene up‐regulation was higher, delayed and more diverse following more severe trauma. Moreover, one of the most up‐regulated genes was periostin, suggestive for severe muscle damage and unfavourable architecture restoration.

中文翻译:

骨骼肌再生早期粘附和细胞外基质分子的基因表达谱。

骨骼肌再生暗示着肌生成与骨髓细胞募集和细胞外基质(ECM)重塑的协调。目前,尚无用于诊断肌肉病变严重程度和预后的特异性生物标志物。为了研究细胞外基质和粘附分子的基因表达谱,作为同种或异种细胞合作和骨骼肌再生的里程碑,我们对小鼠中参与细胞合作,迁移和ECM重塑的基因进行了基因表达分析急性挤压伤模型。将在受伤后两个早期时间点获得的结果与来自其他两个创伤模型的GSE5413数据集进行比较。受伤后第三天,当炎性细胞入侵时,与细胞基质相互作用和迁移相关的基因被上调。第5天后,随着成肌细胞迁移和分化的开始,基底膜成分的基因被下调,而ECM分子,巨噬细胞,成肌细胞粘附和迁移受体的基因被上调。但是,其概况和诱导时间根据实验模型而有所不同,只有极少数基因被不断上调。在更严重的创伤后,基因上调更高,被延迟并且更加多样化。此外,最上调的基因之一是骨膜素,提示严重的肌肉损伤和不利的结构修复。巨噬细胞,成肌细胞粘附和迁移受体被上调。但是,其概况和诱导时间根据实验模型而有所不同,只有极少数基因被不断上调。在更严重的创伤后,基因上调更高,被延迟并且更加多样化。此外,最上调的基因之一是骨膜素,提示严重的肌肉损伤和不利的结构修复。巨噬细胞,成肌细胞粘附和迁移受体被上调。但是,其概况和诱导时间根据实验模型而有所不同,只有极少数基因被不断上调。在更严重的创伤后,基因上调更高,被延迟并且更加多样化。此外,最上调的基因之一是骨膜素,提示严重的肌肉损伤和不利的结构修复。
更新日期:2020-07-18
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