Abstract Yellow mosaic virus (YMV) infection occurred through whitefly (Bemisia tabaci) transmitted begomovirus in black gram during kharif season but the crop did not show any signs of infection during the spring season. To compare and comprehend the intricate link between the glutathione molecule and methylglyoxal detoxification pathway, three black gram cultivars-cv.KUG253 (highly susceptible to YMV), MASH114 (resistant) and cross (cv. KUG253 × MASH114) were sown during kharif and spring season. YMV brutally infected the leaves of cv.KUG253 in the kharif season at 20 days after sowing (DAS) and caused severe chlorosis by 40 DAS which made the leaves papery and feeble. On the contrary, YMV infection was completely absent in the other two cultivars of the black gram and during the spring season, all three cultivars exhibited normal growth without any sign of this disease. The main cytotoxic product formed under YMV infection was methylglyoxal, as significantly higher specific activity was observed for methylglyoxal reductase (MGR) and glyoxalase enzymes in the kharif season rather than spring season in all the three cultivars. Glyoxalase III has been recently discovered for the detoxification of MG and is independent of the glutathione molecule, but no report has been published till now which elaborately explains its role during the pathogenic attack on the plants.

中文翻译：

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谷胱甘肽在黄花叶病毒 (YMV) 感染黑革兰 (Vigna mungo (L.) Hepper) 期间甲基乙二醛解毒途径中的作用

摘要 黄花叶病毒 (YMV) 感染是通过粉虱 (Bemisia tabaci) 在 kharif 季节在黑革兰中传播的 begomovirus 而发生的，但该作物在春季没有任何感染迹象。为了比较和理解谷胱甘肽分子与甲基乙二醛解毒途径之间错综复杂的联系，在 kharif 和春季播种了三个黑革兰品种 - cv.KUG253（对 YMV 高度敏感）、MASH114（抗性）和杂交（cv. KUG253 × MASH114）季节。YMV在播种后20天（DAS）的kharif季节残酷地感染cv.KUG253的叶子，并通过40 DAS导致严重的萎黄病，使叶子变得纸质和虚弱。相反，黑革兰的另外两个品种和春季期间完全没有YMV感染，所有三个品种都表现出正常的生长，没有任何这种疾病的迹象。在 YMV 感染下形成的主要细胞毒性产物是甲基乙二醛，因为在 kharif 季节而不是在所有三个品种的春季，观察到甲基乙二醛还原酶 (MGR) 和乙二醛酶的比活性显着更高。乙二醛酶 III 最近被发现用于 MG 的解毒，并且不依赖于谷胱甘肽分子，但到目前为止还没有发表详细解释其在植物病原侵袭过程中作用的报告。因为在 kharif 季节而不是在所有三个品种的春季，观察到甲基乙二醛还原酶 (MGR) 和乙二醛酶的比活性显着更高。乙二醛酶 III 最近被发现用于 MG 的解毒，并且不依赖于谷胱甘肽分子，但到目前为止还没有发表详细解释其在植物病原侵袭过程中作用的报告。因为在 kharif 季节而不是在所有三个品种的春季，观察到甲基乙二醛还原酶 (MGR) 和乙二醛酶的比活性显着更高。乙二醛酶 III 最近被发现用于 MG 的解毒，并且不依赖于谷胱甘肽分子，但到目前为止还没有发表详细解释其在植物病原侵袭过程中作用的报告。