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Production and antiproliferative effect of violacein, a purple pigment produced by an Antarctic bacterial isolate
World Journal of Microbiology and Biotechnology ( IF 4.1 ) Pub Date : 2020-07-18 , DOI: 10.1007/s11274-020-02893-4
Diego Alem 1, 2 , Juan José Marizcurrena 2 , Verónica Saravia 3 , Danilo Davyt 4 , Wilner Martinez-Lopez 1 , Susana Castro-Sowinski 2, 5
Affiliation  

We studied the production and the potential use of a purple-pigment produced by an Antarctic bacterial isolate. This pigment was identified as violacein, a metabolite produced by many bacterial strains and reported that it has antiproliferative activity in many cell lines. We analyzed the effect of temperature and the composition of the growth medium on pigment production, achieving the highest yield at 20 °C in Tryptic Soy Broth medium supplemented with 3.6 g/L glucose. We doubled the yield of the pigment production when the process was scaled up in a 5 L bioreactor (77 mg/L of crude pigment). The pigment was purified and identified by mass spectrometry (DI–EI–MS) and Nuclear Magnetic Resonance (NMR) spectroscopy as violacein. We performed survival assays that showed that the pure pigment has antiproliferative activity and sensitize HeLa cells (cervix cell carcinoma) to cisplatin. Besides, the pigment did not show genotoxic activity in HeLa cells as found performing micronucleus assays. These results suggest that this pigment may be used as anticancer or sensitizer to cisplatin drug in cervix cancer.

中文翻译:

紫罗兰素的产生和抗增殖作用,一种由南极细菌分离物产生的紫色色素

我们研究了一种由南极细菌分离株产生的紫色颜料的生产和潜在用途。这种色素被鉴定为紫罗兰素,一种由许多细菌菌株产生的代谢物,据报道它在许多细胞系中具有抗增殖活性。我们分析了温度和生长培养基组成对色素生产的影响,在补充 3.6 g/L 葡萄糖的胰蛋白酶大豆肉汤培养基中,在 20 °C 下获得了最高产量。当该工艺在 5 L 生物反应器(77 mg/L 粗色素)中按比例放大时,我们将色素产量提高了一倍。通过质谱 (DI-EI-MS) 和核磁共振 (NMR) 光谱法纯化并鉴定色素为紫罗兰素。我们进行了生存分析,结果表明纯色素具有抗增殖活性并使 HeLa 细胞(宫颈细胞癌)对顺铂敏感。此外,在进行微核试验时发现,该色素在 HeLa 细胞中没有显示出基因毒性活性。这些结果表明该色素可用作抗癌剂或宫颈癌顺铂药物的敏化剂。
更新日期:2020-07-18
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