The world is at a critical stage to switch from fossil and agriculture feedstocks with sustainable alternatives for the production of biobased chemicals of everyday use. This has spurred interest in using carbon one compounds; methanol and formate as a substrate or cosubstrate for microbial-based production. However, considering that native methylotrophs and formatotrophs utilize methanol and formate respectively, their capabilities to efficiently produce high value-added chemicals are limited. Therefore, shifting from these native C1 microbes to metabolically engineered non-native C1 model strains has attracted increasing attention thanks to many advantages such as the availability of well-established tools and strategies for metabolic engineering, and in addition to its high cell growth rate. Herein, we discussed recent trends in developing synthetic methylotrophs and formatotrophs for methanol and formate-based biomanufacturing. Finally, we highlighted barriers and provided broad prospects on possible avenues for optimizing synthetic methylotrophic and formatotrophic strains with respect to the recent advances in biology.

中文翻译：

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工程合成甲基营养菌和形态营养菌的新前景：推进基于 C1 的化学品生产的课程

世界正处于从化石和农业原料转变为生产日常使用的生物基化学品的可持续替代品的关键阶段。这激发了人们对使用碳一化合物的兴趣；甲醇和甲酸盐作为微生物生产的底物或共底物。然而，考虑到天然甲基营养生物和形成营养生物分别利用甲醇和甲酸盐，它们有效生产高附加值化学品的能力是有限的。因此，从这些天然 C1 微生物转移到代谢工程化的非天然 C1 模型菌株已经引起了越来越多的关注，这要归功于许多优势，例如成熟的代谢工程工具和策略的可用性，以及高细胞生长率。在此处，我们讨论了开发用于甲醇和甲酸盐生物制造的合成甲基营养菌和形态营养菌的最新趋势。最后，我们强调了障碍，并根据生物学的最新进展提供了优化合成甲基营养和形态营养菌株的可能途径的广阔前景。