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Application of a Dye-Decolorizing Pseudomonas aeruginosa Strain ZM130 for Remediation of Textile Wastewaters in Aerobic/Anaerobic Sequential Batch Bioreactor and Soil Columns
Water, Air, & Soil Pollution ( IF 2.9 ) Pub Date : 2020-07-17 , DOI: 10.1007/s11270-020-04777-7
Zahid Maqbool , Muhammad Shahid , Farrukh Azeem , Tanvir Shahzad , Faisal Mahmood , Muzammal Rehman , Tanvir Ahmed , Muhammad Imran , Sabir Hussain

In this study, a pre-isolated dye-decolorizing bacterium, Pseudomonas aeruginosa ZM130, was tested to explore its potential for the treatment of synthetic and real textile wastewaters in aerobic and anaerobic bench-scale sequencing batch bioreactors (SBRs) as well as in constructed soil columns. The decolorizing ability of strain ZM130 against reactive black-5 (RB5) was optimized following a response surface methodology (RSM)-based approach, and this strain was observed to effectively decolorize RB5 even in presence of a substantial quantity of NaCl salt along multi-metal mixture including Cr6+, Pb2+, Cd2+, and Zn2+. In the SBR containing the immobilized cells of the strain ZM130, more than 80% of the RB5, hexavalent chromium [Cr(VI)], and chemical oxygen demand (COD) were removed from the textile wastewater under partially anaerobic condition using yeast extract as an additional carbon co-substrate. Furthermore, while studying the bioremediating ability of strain ZM130 in constructed soil columns, the maximum color removal (> 90%), Cr(VI) removal (> 95%), and COD removal (> 90%) were achieved in the soil columns bioaugmented with ZM130 together with either sludge or yeast extract. Interestingly, it was further noticed that soil columns augmented with P. aeruginosa ZM130 also showed maximum color removal from real textile wastewater in vertical columns filled with sterilized (> 87%) and non-sterilized soil (> 91%). Based on the results of the present research work, it can be concluded that P. aeruginosa ZM130 can serve as an excellent potential candidate for treatment of textile wastewaters in bioreactors as well as in soils.



中文翻译:

染料脱色铜绿假单胞菌菌株ZM130在好氧/好氧顺序分批生物反应器和土壤柱中修复纺织废水中的应用

在这项研究中,对预先分离的染料脱色细菌铜绿假单胞菌ZM130进行了测试,以探索其在需氧和厌氧台式规模化批处理生物反应器(SBR)以及已构建的合成和实际纺织废水中的处理潜力。土柱。ZM130菌株对反应性黑5(RB5)的脱色能力根据基于响应表面方法(RSM)的方法进行了优化,并且观察到该菌株即使沿大量NaCl盐存在也能有效使RB5脱色。包括Cr 6+,Pb 2 +,Cd 2+和Zn 2+的金属混合物。在含有ZM130菌株固定化细胞的SBR中,使用酵母提取物作为部分厌氧条件,从纺织废水中去除了80%以上的RB5,六价铬[Cr(VI)]和化学需氧量(COD)。额外的碳共衬底。此外,在研究菌株ZM130在人工土壤柱中的生物修复能力时,在土壤柱中实现了最大的色去除(> 90%),六价铬(> 95%)和COD去除(> 90%)。 ZM130与污泥或酵母提取物一起生物增强。有趣的是,人们还注意到土壤柱中增加了铜绿假单胞菌ZM130还显示了在填充有灭菌(> 87%)和未灭菌土壤(> 91%)的立式色谱柱中,从真实纺织废水中去除的最大色度。根据当前研究工作的结果,可以得出结论,铜绿假单胞菌ZM130可以作为生物反应器和土壤中纺织废水处理的极佳潜在候选者。

更新日期:2020-07-18
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