BES1/BZR1 is a kind of transcription factors in brassinosteroid signal pathway, which play a vital role in plant growth and development, the responses to stresses in many plants, like Arabidopsis and rice. However, there are few reports for BES1 in potato, which is an important crop in China. Here, we identified nine potato BES1 genes by genome-wide analysis, and amplified all these CDS sequences which were localized on eight chromosomes. All StBES1 proteins had conserved domains including bHLH domains, BIN2 phosphorylation sites and nucleus localization sites, and shared similar motif compositions. The gene expression displayed differential in different organs and under different hormone and stress treatments. Subcellular localization showed that all StBES1 proteins localized in nucleus and cytoplasm, and transactivation assay indicated these nine StBES1 proteins played roles as transcription factors. All these results provide a theoretic basis for further studying StBES1 functions in potato.

中文翻译：

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马铃薯 BES1/BZR1 转录因子家族全基因组鉴定与分析

BES1/BZR1是油菜素类固醇信号通路中的一种转录因子，在植物生长发育、拟南芥、水稻等多种植物的逆境响应中发挥重要作用。然而，在我国重要的农作物马铃薯中，关于BES1的报道很少。在这里，我们通过全基因组分析鉴定了九个马铃薯 BES1 基因，并扩增了所有这些位于八条染色体上的 CDS 序列。所有 StBES1 蛋白都具有保守结构域，包括 bHLH 结构域、BIN2 磷酸化位点和细胞核定位位点，并具有相似的基序组成。基因表达在不同器官和不同激素和应激处理下表现出差异。亚细胞定位显示所有 StBES1 蛋白均定位于细胞核和细胞质中，反式激活试验表明这九种 StBES1 蛋白起到了转录因子的作用。这些结果为进一步研究马铃薯中StBES1的功能提供了理论依据。