This study focuses on developing a highly sensitive immunosensor by immobilizing oxidized carbon nanohorns/Nafion/Fe3O4@Pd nanocomposite on carbon screen-printed electrodes (SPEs) for the detection of tropomyosin (Tro-Ag). The performance of the fabricated immunosensor was investigated via electrochemiluminescence (ECL) method, resulting from the chemical reaction between tris(bipyridine)ruthenium(II) chloride ([Ru(bpy)3]Cl2) and tripropylamine (TPrA), and the peak intensity is recorded at 1.0 V. The nanocomposite is able to enhance the ECL intensity of [Ru(bpy)3]2 +/TPrA system and achieves high sensitivity of 28.16 fg/mL with a dynamic working range of 28.16 fg/mL to 100 ng/ml.. Furthermore, the immunosensor demonstrated a decent stability and good repeatability for Tro-Ag detection in food products. Graphical abstract The schematic representation of the modified carbon SPE with CNHs-OH/Nafion/Fe3O4@Pd and the signal produced in the (a) absence and (b) presence of Tro-Ag. The schematic representation of the modified carbon SPE with CNHs-OH/Nafion/Fe3O4@Pd and the signal produced in the (a) absence and (b) presence of Tro-Ag.

中文翻译：

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使用碳纳米角/Nafion/Fe3O4@Pd丝网印刷电极的原肌球蛋白电化学发光免疫传感器

本研究的重点是通过将氧化碳纳米角/Nafion/Fe3O4@Pd 纳米复合材料固定在碳丝网印刷电极 (SPE) 上来开发一种高灵敏度的免疫传感器，用于检测原肌球蛋白 (Tro-Ag)。通过电化学发光（ECL）方法研究制造的免疫传感器的性能，由三（联吡啶）氯化钌（II）（[Ru（bpy）3]Cl2）和三丙胺（TPrA）之间的化学反应产生，峰强度在 1.0 V 下记录。纳米复合材料能够增强 [Ru(bpy)3]2 +/TPrA 系统的 ECL 强度并实现 28.16 fg/mL 的高灵敏度，动态工作范围为 28.16 fg/mL 至 100 ng /ml.. 此外，免疫传感器在食品中的 Tro-Ag 检测中表现出良好的稳定性和良好的可重复性。图形摘要 具有 CNHs-OH/Nafion/Fe3O4@Pd 的改性碳 SPE 的示意图以及在 (a) 不存在和 (b) Tro-Ag 存在下产生的信号。具有 CNHs-OH/Nafion/Fe3O4@Pd 的改性碳 SPE 的示意图以及在 (a) 不存在和 (b) Tro-Ag 存在下产生的信号。