Sample preparation including dehydration and drying of samples is the most intricate part of scanning electron microscopy. Most current sample preparation protocols use critical-point drying with liquid carbon dioxide. Very few studies have reported samples that were dried using chemical reagents. In this study, we used hexamethyldisilazane, a chemical drying reagent, to prepare plant samples. As glandular trichomes are among the most fragile and sensitive surface structures found on plants, we used Millingtonia hortensis leaf samples as our study materials because they contain abundant glandular trichomes. The results obtained using this new method are identical to those produced via critical-point drying.

中文翻译：

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用低成本化学干燥代替临界点干燥提供了来自 Millingtonia hortensis L. f. 叶子的腺毛状体的可比表面图像质量。在扫描电子显微照片中

样品制备，包括样品的脱水和干燥，是扫描电子显微镜中最复杂的部分。大多数当前的样品制备方案使用液态二氧化碳进行临界点干燥。很少有研究报告使用化学试剂干燥的样品。在本研究中，我们使用六甲基二硅氮烷（一种化学干燥剂）来制备植物样品。由于腺毛是植物上发现的最脆弱和最敏感的表面结构之一，我们使用 Millingtonia hortensis 叶样本作为我们的研究材料，因为它们含有丰富的腺毛。使用这种新方法获得的结果与通过临界点干燥产生的结果相同。