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In vitro estimation of superfluid critical extracts of some plants for their antimicrobial potential, phytochemistry, and GC-MS analyses.
Annals of Clinical Microbiology and Antimicrobials ( IF 5.7 ) Pub Date : 2020-07-17 , DOI: 10.1186/s12941-020-00371-1
Waleed Bakry Suleiman 1, 2
Affiliation  

Along with swift economic evolution and continuous amelioration of lifestyle, people at present are paying more attention to health issues. Synthetic drugs will be compensated with other natural ones that belong to natural origin. Plants have always been considered as sources of several compounds that are used in many fields, especially human and animal health, starting from boosting immunity to the treatment of infectious diseases caused by some pathogenic microbes such as bacteria, fungi as well as viruses. This study aimed to incorporate some types of plants within the antimicrobial portfolio through the examination of different six plants which were Cichorium intybus, Cinnamomum camphora, Commiphora myrrha, Foeniculum vulgare, Nerium oleander, and Spartium junceum. As well, attempting to identify the active constituents of their extracts using GC–MS. All selected plants were analyzed to determine their phytochemical composition such as phenolics, alkaloids, flavonoids, terpenoids, and so on. The extraction step was done by sophisticated equipment called supercritical fluid extractor SFE through adjustment of specific conditions include temperature, time, flow rate and pressure to change the behavior of CO2. Testing the antimicrobial activity of each plant extract via agar well diffusion method through the formation of clear zones against a wide range of test microorganisms including both Gram-positive and Gram-negative bacteria as well as yeasts. Finally, attempting to primarily identify the constituents of each plant extract using GC–MS. The crude extract of F. vulgare showed the highest potency against C. albicans, E. faecalis and S. typhimurium, it contains some unique compounds such as squalene, eugenol and isoeugenol while, Extract of C. intybus showed a moderate activity especially against C. lipolytica and MRSA and it includes Vitamin A like compound which indicates antioxidant property. Conclusively, fennel gave a promising result as a good wide spectrum antimicrobial agent because it contains some compounds act as antimicrobial agents such as eugenol which was used as food preservatives in addition to squalene which acts as an antioxidant and antimycotic agent so, it will be useful especially while it was used in highly purified form excluding all undesirable subcomponents.

中文翻译:

体外评估某些植物的超流临界提取物的抗菌潜力,植物化学和GC-MS分析。

随着经济的飞速发展和生活方式的不断改善,目前人们越来越关注健康问题。合成药物将获得其他天然来源的天然药物的补偿。从增强免疫力到治疗由某些病原微生物(例如细菌,真菌和病毒)引起的传染病开始,植物一直被认为是用于许多领域(尤其是人类和动物健康)的几种化合物的来源。这项研究的目的是通过检查六种不同的植物来将某些类型的植物纳入抗菌组合中,这六种植物是菊苣菊,樟脑,樟子松,大叶茴香,夹竹桃和君子草。还有 尝试使用GC-MS鉴定其提取物中的活性成分。分析所有选定的植物以确定其植物化学组成,例如酚类,生物碱,类黄酮,萜类化合物等。萃取步骤由称为超临界流体萃取器SFE的复杂设备通过调节特定条件(包括温度,时间,流速和压力以改变CO2的行为)来完成。通过琼脂井扩散法,通过形成透明区,针对各种测试微生物(包括革兰氏阳性细菌和革兰氏阴性细菌以及酵母菌),测试每种植物提取物的抗菌活性。最后,尝试使用GC-MS初步鉴定每种植物提取物的成分。F. vulgare的粗提物显示出对白色念珠菌的最高效力,粪肠球菌和鼠伤寒沙门氏菌中含有一些独特的化合物,例如角鲨烯,丁子香酚和异丁香酚,而茵茵的提取物显示出中等的活性,尤其是对解脂丙酸杆菌和MRSA的活性,并且它含有维生素A类化合物,表明其具有抗氧化性能。最终,茴香作为一种良好的广谱抗菌剂而获得了令人鼓舞的结果,因为它除了用作抗氧化剂和抗霉菌剂的角鲨烯外,还包含一些用作抗菌剂的化合物,例如丁香酚,用作食品防腐剂,因此将是有用的。特别是当它以高度纯化的形式使用时,不包括所有不希望的亚组分。intybus表现出中等的活性,尤其是对解脂梭状芽胞杆菌和MRSA的活性,并且它包含类似维生素A的化合物,具有抗氧化作用。最终,茴香作为一种良好的广谱抗菌剂而获得了令人鼓舞的结果,因为它除了用作抗氧化剂和抗霉菌剂的角鲨烯外,还包含一些用作抗菌剂的化合物,例如丁香酚,用作食品防腐剂,因此将是有用的。特别是当它以高度纯化的形式使用时,不包括所有不希望的亚组分。intybus表现出中等的活性,尤其是对解脂梭状芽胞杆菌和MRSA的活性,并且它包含类似维生素A的化合物,具有抗氧化作用。最终,茴香作为一种良好的广谱抗菌剂而获得了令人鼓舞的结果,因为它除了用作抗氧化剂和抗霉菌剂的角鲨烯外,还包含一些用作抗菌剂的化合物,例如丁香酚,用作食品防腐剂,因此将是有用的。特别是当它以高度纯化的形式使用时,不包括所有不希望的亚组分。
更新日期:2020-07-17
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