Abstract

Ancient human remains have been assigned to their mitochondrial DNA (mtDNA) haplogroups. To obtain efficiently deep and reliable nucleotide sequences of ancient DNA of interest, we achieved target enrichment followed by next-generation sequencing (NGS). Complete mitochondrial genome (mitogenome) sequences were obtained for three human remains from the Iyai rock-shelter site of the Initial Jomon Period in Japan. All the Jomon mitogenomes belong to haplogroup N9b, but no sequences among them were identical. High genetic diversity was clarified even among the Jomon human remains belonging to haplogroup N9b, which has been described as a haplogroup representing the Jomon people.

摘要

古代人类遗骸已被分配给其线粒体DNA（mtDNA）单倍群。为了有效地获得感兴趣的古代DNA的深度和可靠核苷酸序列，我们实现了目标富集，然后进行了下一代测序（NGS）。从日本绳纹初期的Iyai岩石庇护所获得了三个人类遗体的完整线粒体基因组（mitogenome）序列。所有绳纹线虫基因组均属于单倍型N9b，但其中没有序列是相同的。即使属于单倍群N9b的绳纹人类遗骸中也已阐明了高度的遗传多样性，后者被描述为代表绳纹人的单倍群。