Abstract

Species from the Genus Calophyllum are rich source for bioactive phenolic compounds such as coumarins and xanthones. Phytochemical study carried out on the plant, Calophyllum macrocarpum has led to the isolation of three known prenylated xanthones, ananixanthone (1), trapezifolixanthone (2) and 8-deoxygartanin (3) with two common triterpenoids, stigmasterol (4), and friedelin (5). The structures of these compounds were identified and determined using spectroscopic techniques such as NMR and MS. The cytotoxic activities of compounds 1 and 2 as well as the extracts were tested against HeLa Chang liver and HEK-293 cell lines. Compound 1 exhibited appreciable cytotoxicity with the IC₅₀ value of 11.08 ± 3.09 µM against HeLa Chang liver cell line and moderate cytotoxicity against HEK-293 cell line while compound 2 showed limited toxicity against these two cell lines.

摘要

Calophyllum属的物种富含生物活性酚类化合物，如香豆素和氧杂蒽酮。对植物Calophyllum macrocarpum进行的植物化学研究导致分离出三种已知的异戊二烯化的氧杂蒽酮，即茴香蒽酮 ( 1 )、梯形叶黄酮 ( 2 ) 和 8-脱氧花叶苷 ( 3 )，以及两种常见的三萜类化合物豆甾醇 ( 4 ) 和 Friedelin ( 4)。 5）。这些化合物的结构通过NMR和MS等光谱技术进行鉴定和确定。化合物1和2的细胞毒活性以及提取物针对 HeLa Chang 肝和 HEK-293 细胞系进行了测试。化合物1表现出明显的细胞毒性，对HeLa Chang肝细胞系的IC ₅₀值为11.08 ± 3.09 µM，对HEK-293细胞系有中等的细胞毒性，而化合物2对这两种细胞系的毒性有限。