Abstract

The present study investigated the possibility of apoptosis-inducing activity in human leukemia U-937 and THP-1 cells by the flavonoid morin. The treatments were evaluated by using the MTT and LDH assays; analysis of mitochondrial membrane potential (ΔΨm) was evaluated by flow cytometry, cell death by apoptosis was confirmed by fluorescence microscopy and by assessing the activity of caspases-3 and −6. The data indicated that the flavonoid morin has promoted a decrease in cell viability in a concentration-dependent way for both of the cancerous cell lines. An increase in the percentage of cell death caused by apoptosis was associated to a potential alteration in the mitochondrial membrane (ΔΨm) suggesting the involvement of cell death in intrinsic apoptotic pathways. Activation of caspases-3 and −6 confirmed the presence of apoptotic activity from morin. The results reinforce the antileukemic potential of flavonol morin.

摘要

本研究调查了黄酮类桑椹素在人白血病 U-937 和 THP-1 细胞中诱导细胞凋亡的可能性。通过使用MTT和LDH测定来评估处理；通过流式细胞术评估线粒体膜电位 (ΔΨm) 的分析，通过荧光显微镜和评估 caspase-3 和 -6 的活性证实细胞凋亡导致的细胞死亡。数据表明，黄酮类桑椹素以浓度依赖的方式促进了两种癌细胞系的细胞活力降低。由细胞凋亡引起的细胞死亡百分比的增加与线粒体膜 (ΔΨm) 的潜在改变有关，表明细胞死亡参与了内在的细胞凋亡途径。caspase-3 和 -6 的激活证实了来自 Morin 的细胞凋亡活性的存在。结果加强了黄酮醇桑椹素的抗白血病潜力。