Balanophora involucrata J. D. Hooker has been known to possess potential anti‐inflammatory and antibacterial activities; however, its antiviral activity has not been evaluated so far. In order to find new neuraminidase inhibitors (NAIs), the neuraminidase (NA) inhibition activity of different B. involucrata extracts was evaluated. In this study, an in vitro NA inhibition assay was performed to identify which extract of B. involucrata exhibits (maximal) inhibitory activity against NA. Ultra high performance liquid chromatography/quadrupole time‐of‐flight–tandem mass spectroscopy (MS/MS) and molecular docking techniques were used to identify the specific compounds responsible for the anti‐influenza activity of the extract, and to explore the potential natural NAIs. The ethyl acetate extract of B. involucrata exhibited significant inhibitory activity against NA with 50% inhibitory concentration (IC₅₀) value of 159.5 μg/mL. Twenty compounds were identified according to the MS/MS spectra; among them two compounds (quercitrin and phloridzin) showed obvious inhibitory activity against NA, with IC₅₀ of 311.76 and 347.32 μmol/L, respectively. This study suggested that B. involucrata can be a potential natural source of NAIs and may be useful in the fight against ferocious influenza viruses.

中文翻译：

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Balanophora involucrata化学成分的抗神经氨酸酶活性。

众所周知，Balanophora involucrata JD Hooker具有潜在的抗炎和抗菌活性。但是，迄今为止尚未评估其抗病毒活性。为了找到新的神经氨酸酶抑制剂（NAIs），评估了不同的B. involucrata提取物对神经氨酸酶（NA）的抑制活性。在这项研究中，进行了体外NA抑制测定，以鉴定哪一种B. involucrata提取物对NA显示（最大）抑制活性。超高效液相色谱/四极杆飞行时间-串联质谱（MS / MS）和分子对接技术用于鉴定导致提取物抗流感活性的特定化合物，并探索潜在的天然NAI 。球形芽孢杆菌的乙酸乙酯提取物显示出对NA的显着抑制活性，其50％抑制浓度（IC ₅₀）值为159.5μg/ mL。根据MS / MS光谱鉴定出二十种化合物；其中两种化合物（槲皮素和Phloridzin）对NA有明显的抑制作用，IC _50分别为311.76和347.32μmol/ L。这项研究表明，B。involucrata 可能是NAI的潜在天然来源，并且可能有助于对抗凶猛的流感病毒。