Coccinia grandis is an important food crop of the Cucurbitaceae family, widely used for culinary purposes in India. It is reported to possess hypoglycemic, hypolipidemic and antioxidant activities. The current study was aimed to explore the inhibition kinetics as well as major constituents of the active fraction of C. grandis against α‐glucosidase. The kinetic study was performed through spectrophotometric assay, with p‐nitrophenyl‐α‐d‐glucopyranoside as a substrate with varying concentrations. An in vitro antioxidant study was performed by DPPH assay. In addition, UPLC–QTOF–MS analysis was carried out for metabolite profiling of the bioactive fraction of C. grandis. The results showed that the difference between the α‐glucosidase inhibitory activity of the ethyl acetate fraction of C. grandis (EFCG) (IC₅₀ 2.43 ± 0.27 mg/ml), and standard inhibitor, acarbose (2.08 ± 0.19 mg/ml), was not statistically significant at a P‐value of 0.05. The enzyme kinetics confirmed the inhibition mode in a mixed manner. The EFCG also showed the highest antioxidant activity (101.74 ± 1.95 μg/ml) among all of the fractions. A significant correlation between antioxidant and α‐glucosidase inhibitory activity of EFCG was observed. The LC–QTOF–MS study of the EFCG putatively identified 35 metabolites, which may be responsible for its antioxidant and α‐glucosidase inhibitory properties. Thus, C. grandis fruits can serve as a functional food to address diabetes‐related disorders associated with α‐glucosidase.

中文翻译：

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基于LC-QTOF-MS的代谢产物分析和大球藻果实α-葡萄糖苷酶抑制动力学评估。

巨球藻是葫芦科的重要粮食作物，在印度被广泛用于烹饪。据报道具有降血糖，降血脂和抗氧化活性。当前的研究旨在探索巨球藻对α-葡萄糖苷酶的抑制动力学以及主要成分。通过分光光度法进行动力学研究，以对硝基苯基-α - d-吡喃葡萄糖苷为底物，浓度不同。的体外抗氧化剂的研究是由DPPH法进行。此外，还进行了UPLC–QTOF–MS分析，对代谢产物的生物活性成分进行了分析。C. grandis。结果表明，所述的差α葡萄糖苷酶的乙酸乙酯馏分的抑制活性C.桉（EFCG）（IC ₅₀ 2.43±0.27毫克/毫升），和标准的抑制剂，阿卡波糖（2.08±0.19毫克/毫升），P值为0.05时，无统计学意义。酶动力学以混合方式证实了抑制模式。在所有馏分中，EFCG还显示出最高的抗氧化活性（101.74±1.95μg/ ml）。观察到EFCG的抗氧化剂和α-葡萄糖苷酶抑制活性之间存在显着相关性。EFCG的LC–QTOF–MS研究确定了35种代谢物，可能是其抗氧化剂和α的原因葡糖苷酶抑制特性。因此，C。grandis果实可以作为功能性食品来解决与α-葡萄糖苷酶相关的糖尿病相关疾病。