Neurophysiological recordings were employed to quantify neuronal sensitivity to neurotoxic insecticides and assessed toxicity across field and laboratory fall armyworm (FAW) populations. Topical toxicity resistance ratios (RR) in field-collected FAW was 767-fold compared to laboratory strains and, importantly, a 1750-fold reduction in potency was observed for λ-cyhalothrin in neurophysiological assays. Field collected FAW were found to have a RR of 12 to chlorpyrifos when compared to the susceptible strain and was 8-fold less sensitive in neurophysiological assays. Surprisingly, there were no point mutations identified in the voltage-gated sodium channel known to cause pyrethroid resistance. For acetylcholinesterase, FAW had more than 80% of their nucleotide sequences consistent with A201 and F290 of the susceptible strains although 60% of the tested population was heterozygous for the G227A mutation. These data indicate that point mutations did not contribute to the high level of pyrethroid resistance and nerve insensitivity in this population of field collected FAW. Additionally, these data suggest the kdr phenotype only explains a portion of the heritable variation in FAW resistance and indicates kdr is not the only predictor of high pyrethroid resistance. Phenotypic assays, such as toxicity bioassays or neurophysiological recordings, using field-collected populations are necessary to reliably predict resistant phenotypes and product failures.

中文翻译：

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在没有已知敲低突变的情况下，秋季粘虫草地贪夜蛾对拟除虫菊酯的神经元敏感性和易感性降低

神经生理学记录用于量化神经元对神经毒性杀虫剂的敏感性，并评估田间和实验室秋粘虫 (FAW) 种群的毒性。与实验室菌株相比，野外收集的秋粘虫的局部毒性抗性比 (RR) 是 767 倍，重要的是，在神经生理学测定中观察到 λ-三氯氟氰菊酯的效力降低了 1750 倍。与敏感菌株相比，现场收集的秋粘虫对毒死蜱的 RR 为 12，在神经生理学检测中的敏感性低 8 倍。令人惊讶的是，在已知会导致拟除虫菊酯抗性的电压门控钠通道中没有发现点突变。对于乙酰胆碱酯酶，FAW 有超过 80% 的核苷酸序列与敏感菌株的 A201 和 F290 一致，尽管 60% 的测试人群是 G227A 突变的杂合子。这些数据表明，点突变不会导致这一领域收集的 FAW 群体中拟除虫菊酯的高水平抗性和神经不敏感。此外，这些数据表明 kdr 表型仅解释了 FAW 抗性的部分遗传变异，并表明 kdr 不是高拟除虫菊酯抗性的唯一预测因子​​。表型分析，如毒性生物分析或神经生理学记录，使用现场收集的种群对于可靠地预测抗性表型和产品失败是必要的。这些数据表明，点突变不会导致这一领域收集的 FAW 群体中拟除虫菊酯的高水平抗性和神经不敏感。此外，这些数据表明 kdr 表型仅解释了 FAW 抗性的部分遗传变异，并表明 kdr 不是高拟除虫菊酯抗性的唯一预测因子​​。表型分析，如毒性生物分析或神经生理学记录，使用现场收集的种群对于可靠地预测抗性表型和产品失败是必要的。这些数据表明，点突变不会导致这一领域收集的 FAW 群体中拟除虫菊酯的高水平抗性和神经不敏感。此外，这些数据表明 kdr 表型仅解释了 FAW 抗性的部分遗传变异，并表明 kdr 不是高拟除虫菊酯抗性的唯一预测因子​​。表型分析，如毒性生物分析或神经生理学记录，使用现场收集的种群对于可靠地预测抗性表型和产品失败是必要的。