Effects of acute oral lead exposure on the levels of essential elements of mice: a metallomics and dose-dependent study.,Journal of Trace Elements in Medicine and Biology

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Effects of acute oral lead exposure on the levels of essential elements of mice: a metallomics and dose-dependent study.
Journal of Trace Elements in Medicine and Biology ( IF 3.5 ) Pub Date : 2020-07-17 , DOI: 10.1016/j.jtemb.2020.126624
Yaqi Yu ₁ , Leilei Yu ₂ , Xingting Zhou ₁ , Nanzhen Qiao ₁ , Dingwu Qu ₁ , Fengwei Tian ₂ , Jianxin Zhao ₃ , Hao Zhang ₄ , Qixiao Zhai ₂ , Wei Chen ₅

Affiliation

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; International Joint Research Laboratory for Probiotics at Jiangnan University, Wuxi, Jiangsu, 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, 225004, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu, 214122, China; (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, 225004, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu, 214122, China; (Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, 225004, China; Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology & Business University, Beijing, 100048, China.

Background and objectives

Lead (Pb) has been reported to disturb the metabolism of essential elements, such as calcium (Ca), magnesium (Mg), iron (Fe) and zinc (Zn) in vivo. This study focused on the relationship between various dose of Pb and the essential elements.

Methods

50 healthy male C57BL/6 mice underwent oral administration of 0.2 mL lead acetate trihydrate solution (0, 20, 100, 500, and 1000 mg Pb/day/kg body weight) for 3 days. The concentrations of Pb and four essential elements (Ca, Zn, Fe and Mg) in the blood, kidney, liver, bone and brain were quantified with inductively coupled plasma mass spectrometry.

Results

Various doses of Pb led to significant increases in the contents of Ca, Fe and Zn in the liver, and decreased contents of Mg and Fe in the blood in a dose-dependent pattern. The Pb dose of 20 mg/kg reduced the concentration of bone Ca, which did not continue to show an obvious decline with continued increases in the oral Pb dose. Pb also caused alterations in the Mg distribution pattern, and decreased the correlation of Mg, Ca and Zn in the brain, both findings were dose-dependent. In addition to the changes in metallomics, the related oxidative stress was exacerbated, but no significant changes were detected in hepatic and renal histopathological lesions after a short period of Pb exposure.

Conclusions

This study contributes to a thorough analysis of the Pb-poisoning mechanism, and indicates that the concentrations of essential elements could be used as sensitive toxicological indicators of Pb exposure.

中文翻译：

急性口服铅暴露对小鼠必需元素水平的影响：金属组学和剂量依赖性研究。

背景和目标

据报道，铅 (Pb) 会干扰体内必需元素的代谢，例如钙 (Ca)、镁 (Mg)、铁 (Fe) 和锌 (Zn)。本研究侧重于不同剂量铅与必需元素之间的关系。

方法

50 只健康雄性 C57BL/6 小鼠口服 0.2 毫升三水醋酸铅溶液（0、20、100、500 和 1000 毫克铅/天/公斤体重）3 天。使用电感耦合等离子体质谱法对血液、肾脏、肝脏、骨骼和大脑中的 Pb 和四种必需元素（Ca、Zn、Fe 和 Mg）的浓度进行定量。

结果

不同剂量的铅导致肝脏中钙、铁和锌的含量显着增加，血液中镁和铁的含量呈剂量依赖性降低。20 mg/kg的Pb剂量降低了骨Ca的浓度，随着口服Pb剂量的持续增加，骨钙浓度没有继续显示出明显的下降。铅还引起镁分布模式的改变，并降低了大脑中镁、钙和锌的相关性，这两个发现都是剂量依赖性的。除了金属组学的变化外，相关氧化应激加剧，但在短期接触铅后肝肾组织病理学病变未检测到显着变化。