Abnormal expression of enzymes involved in epigenetic mechanisms, such as DNA methyl transferases, can trigger large chaos in cellular gene expression networks and eventually lead to cancer progression. In our study, which is a pioneer in the literature that clinicopathologically evaluates the expression of 30 epi-miRNAs in prostate cancer (PCa), we investigated which of the new miRNA class epi-miRNAs could be an effective biomarker in the diagnosis and progression of PCa. In this study, the expression levels of 30 epi-miRNAs in whole blood samples from 25 control, 25 PCa and 40 metastatic PCa patients were investigated by the Quantitative Real-Time PCR method. Then, promoter methylation levels of 11 epi-miRNAs, whose expression levels were found to be significantly higher, were examined by methylation-specific qPCR method. The correlations between miRNA expression levels and clinicopathological parameters (Gleason Score (GS), PSA levels, TNM Staging) in different stages of PCa groups as well as disease-specific expression levels were examined. We found a hypomethylation in the promoter regions of miRNAs that showed a direct proportional increase with PSA levels (miR-34b/c, miR-148a, miR-152), GS’s (miR-34a-5p, miR-34b/c, miR-101-2, miR-126, miR-148a, miR- 152, miR-185-5p) and T staging (miR-34a-5p, miR-34b/c, miR-101-2, miR-126, miR-140, miR-148a, miR-152, miR-185-5p) (p < 0.05). When miR-200a/b was evaluated according to clinicopathological parameters, it acted as an onco-miR in local/local advanced PCa and as a tumor-suppressor-miR in metastatic stage. This study is novel in the sense that our findings draw attention to the important role of miRNAs as diagnostic and prognostic biomarkers in PCa.

表观遗传机制中涉及的酶（例如DNA甲基转移酶）的异常表达会引发细胞基因表达网络的混乱，并最终导致癌症进展。在我们的研究中，这是临床病理学评估前列腺癌（PCa）中30种epi-miRNA表达的文献的开创者，我们调查了哪种新的miRNA类epi-miRNA可以作为诊断和治疗进展的有效生物标志物。 PCa。在这项研究中，通过定量实时PCR方法研究了来自25名对照，25名PCa和40名转移性PCa患者的全血样品中30种epi-miRNA的表达水平。然后，通过甲基化特异性qPCR方法检查了11种epi-miRNA的启动子甲基化水平，发现其表达水平明显更高。检查了PCa组不同阶段中miRNA表达水平与临床病理参数（格里森评分（GS），PSA水平，TNM分期）之间的相关性以及疾病特异性表达水平。我们在miRNA的启动子区域发现了甲基化程度低下的现象，该水平与PSA（miR-34b / c，miR-148a，miR-152），GS（miR-34a-5p，miR-34b / c，miR -101-2，miR-126，miR-148a，miR-152，miR-185-5p）和T分期（miR-34a-5p，miR-34b / c，miR-101-2，miR-126，miR -140，miR-148a，miR-152，miR-185-5p）（p  ＜0.05）。当根据临床病理学参数评估miR-200a / b时，它在局部/局部晚期PCa中起癌基因miR的作用，在转移阶段起肿瘤抑制物miR的作用。从我们的发现引起人们对miRNA在PCa中作为诊断和预后生物标志物的重要作用的关注这一意义上来说，这项研究是新颖的。