Tissue engineering using adipose derived stem cells (ASCs) has become one of the most promising treatments for defective articular cartilage owing to the stability and dynamic differentiation of ASCs. In this study, we fabricated a 3D hybrid scaffold using poly(ε-caprolactone) (PCL) to support the mechanical properties of the regenerating auricle cartilage, and injected a cell-laden alginate hydrogel, containing a mixture of ASCs and chondrocytes, into the PCL scaffold. Using the cell-laden 3D auricle structure, the in vitro chondrogenesis of the ASCs with and without the presence of chondrocytes was examined. Additionally, the feasibility of utilizing the 3D cell-laden auricle structure for cartilage tissue engineering was evaluated in a rat model. In our in vitro and in vivo experiments, we observed that as the ASCs were co-cultured with the chondrocytes, chondrogenic differentiation was encouraged, and the regeneration of cartilage was significantly increased.

由于脂肪干细胞的稳定性和动态分化，使用脂肪衍生干细胞（ASC）进行组织工程已成为关节软骨缺损最有前途的治疗方法之一。在这项研究中，我们使用聚（ε-己内酯）（PCL）制造了3D混合支架，以支持再生的耳廓软骨的机械特性，并将含有ASC和软骨细胞混合物的载有藻酸盐的水凝胶注入细胞中。 PCL支架。使用充满细胞的3D耳廓结构，检查了有或没有软骨细胞存在的ASC的体外软骨形成。此外，在大鼠模型中评估了将3D细胞负载耳廓结构用于软骨组织工程的可行性。在我们的体外和在体内实验中，我们观察到，由于ASC与软骨细胞共培养，促进了软骨分化，并且软骨的再生显着增加。