Lignocellulosic materials require pretreatment to remove lignin enabling the enzyme access to the cellulose. This work used multivariate analysis to investigate the acid and alkali pretreatments of vine pruning followed by enzymatic hydrolysis. The best acid pretreatment conditions were H₂SO₄ 1.5%, 120 °C for 30 min, removing 68.7% of hemicellulose, enabling 95.8% of cellulose recovery. However, this treatment was not enough to allow the enzyme hydrolysis. A second step of treatment with NaOH 3.0% at 120 °C without agitation for 60 min led to a material with 75.0% of cellulose and 25.0% of lignin. However, the lowest glucose yield (80.86% and 32.26 g L⁻¹ of glucose) was obtained after the enzyme hydrolysis of this material. The highest glucose yield (98.72% with 35.06 g L⁻¹) was obtained using a pretreated material containing 68.1% of cellulose and 31.9% of lignin obtained after a milder condition (NaOH 2% at 100 °C), thus showing that not all the lignin need to be removed to obtain a high saccharification yield. A less severe pretreatment with no adverse effect on the glucose yield with the advantage of preserving the non-cellulose biomass fractions was effective for vine prune valorization.

木质纤维素材料需要进行预处理以去除木质素，从而使酶能够进入纤维素。这项工作使用多变量分析来研究葡萄树修剪的酸和碱预处理，然后进行酶促水解。最好酸预处理条件为ħ ₂ SO ₄ 1.5％，120℃下30分钟，除去半纤维素68.7％，使纤维素回收的95.8％。但是，这种处理不足以使酶水解。在120℃下用3.0％的NaOH在不搅拌的情况下处理60分钟的第二步得到具有75.0％的纤维素和25.0％的木质素的材料。但是，最低的葡萄糖产量（80.86％和32.26 g L ^-1该物质经酶水解后得到葡萄糖的葡萄糖。在温和条件下（100°C时为NaOH 2％）得到的预处理材料含有68.1％的纤维素和31.9％的木质素，可以得到最高的葡萄糖产率（98.72％，35.06 g L ^-1）。需要除去木质素以获得高糖化产率。较不严重的预处理对葡萄糖产量无不利影响，其优点是保留了非纤维素生物质组分，对葡萄树修剪增值有效。