Gardnerella vaginalis produces vaginolysin (VLY), a cholesterol-dependent cytolysin, responsible of the cellular lysis and epithelial damage. In recently years, IgY antibodies produced in hens present several uses in diagnostic, treatment and research, however they present the disadvantage of unspecific recognition, which could be improved by the preselection of the epitope based on in silico analysis. Finally, multiantigenic peptides with eight ramification (MAP8) are groups of identical peptides synthesized on polylylisine core that increase immunogenicity, and allows its use to improve in vivo antibodies production and troubleshooting. In this work, we designed and administrated a MAP8 synthetic peptide in a hen to test its ability to induce the production of IgY antibodies. Further, we tested the reactivity of anti-VLY IgY antibody (derived of VLY-MAP8 peptide administration) in detecting the denaturing and native cognate antigen in the complete bacteria by ELISA, dot blot, western blot and inhibition of hemagglutination assay. Results showed the induction of IgY antibodies in the immunized hen, that were able to recognize the native and denatured cognate antigen in samples of culture supernatant and complete bacteria of Gardnerella vaginalis ATCC 14018 strain. Additionally, anti-VLY IgY antibody inhibit > 90% erythrocyte’s lysis. In conclusion, the MAP8 peptide and the produced anti-VLY IgY antibody could serve as a potential diagnostic tool in bacterial vaginosis. Also, our study proposed a method for the improvement of IgY antibodies production supported by the administration of MAP8 peptides selected by in silico analysis of the complete antigen.

阴道加德纳菌产生血管溶素（VLY），这是一种胆固醇依赖性细胞溶素，负责细胞溶解和上皮细胞损伤。近年来，母鸡中产生的IgY抗体在诊断，治疗和研究中有多种用途，但是它们呈现出非特异性识别的缺点，这可以通过基于计算机分析的表位的预选来改善。最后，具有八种分支作用的多抗原肽（MAP8）是在聚赖氨酸核心上合成的同一组肽，它们增加了免疫原性，并可以用于改善体内抗体的产生和故障排除。在这项工作中，我们设计并管理了一只母鸡中的MAP8合成肽，以测试其诱导IgY抗体产生的能力。进一步，我们测试了抗VLY IgY抗体（源自VLY-MAP8肽）在通过ELISA，斑点印迹，蛋白质印迹和血凝凝集抑制检测完整细菌中的变性抗原和天然同源抗原中的反应性。结果显示，在免疫母鸡中诱导了IgY抗体，该抗体能够识别培养上清液和完整的细菌样品中的天然和变性同源抗原。阴道加德纳菌ATCC 14018菌株。此外，抗VLY IgY抗体可抑制90％以上的红细胞溶解。总之，MAP8肽和产生的抗VLY IgY抗体可作为细菌性阴道病的潜在诊断工具。同样，我们的研究提出了一种方法，该方法通过施用通过对完整抗原进行计算机分析而选择的MAP8肽来改善IgY抗体的产生。