Visible light-driven H₂ production contributes to an efficient solar energy conversion. Here, we demonstrate a visible light-driven, mediator-free H₂ production system by coupling a photosensitizer and a genetically engineered whole-cell biocatalyst: Escherichia coli expressing clostridial [FeFe]-hydrogenase and maturase genes. The system consists of three components: a photosensitizer, eosin Y; an electron donor, triethanolamine; and the E. coli whole-cell biocatalyst. Under light irradiation, the whole-cell-based system formed H₂ without an additional electron mediator and achieved a high apparent quantum yield at 530 nm (over 10%). This whole-cell-based system provides simple, efficient, and clean H₂ production.

中文翻译：

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通过光敏剂和全细胞生物催化剂的组合，产生可见光驱动的无外部介体的H2：表达[FeFe]-加氢酶和成熟酶基因的大肠杆菌

可见光驱动的H ₂产生有助于有效的太阳能转换。在这里，我们通过耦合光敏剂和基因工程全细胞生物催化剂展示了可见光驱动的无介质H ₂生产系统：表达梭菌[FeFe]氢化酶和成熟酶基因的大肠杆菌。该系统由三部分组成：光敏剂曙红Y；和 电子给体三乙醇胺；和大肠杆菌全细胞生物催化剂。在光照射下，基于全细胞的系统在没有其他电子介体的情况下形成了H ₂，并在530 nm处实现了较高的表观量子产率（超过10％）。这种基于全细胞的系统可提供简单，高效，清洁的H₂生产。